English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/153635
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Quasi-simultaneous imaging/pulling analysis of single polyprotein molecules by atomic force microscopy

AutorValbuena, A.; Oroz, J.; Vera, A.M.; Gimeno, A.; Gómez-Herrero, J.; Carrión-Vázquez, Mariano Sixto
Fecha de publicación2007
EditorAmerican Institute of Physics
CitaciónReview of Scientific Instruments 78 (2007)
ResumenMost of studies in protein nanomechanics have used the atomic force microscope (AFM) in its force-measuring mode on immobilized protein repeats (polyproteins) as single-molecule markers. Here, we add imaging capabilities to a standard, state-of-the-art AFM >puller> and integrate the most powerful programs of analysis available for both AFM modes. This unique instrument allows high-resolution, quasi-simultaneous imaging/force spectroscopy in aqueous solution. We demonstrate its capabilities using polyproteins of a model system (titin I27 domain). This tool should greatly facilitate the development of a much needed universal functionalization system for AFM, one that should allow better sample control and an improved efficiency of protein immobilization. © 2007 American Institute of Physics.
URIhttp://hdl.handle.net/10261/153635
DOI10.1063/1.2794732
Identificadoresdoi: 10.1063/1.2794732
issn: 0034-6748
Aparece en las colecciones: (IC) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.