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Gain-of-function mutations in Aqp3a influence zebrafish pigment pattern formation through the tissue environment

AutorEskova, Anastasia; Chauvigné, François ; Maischein, Hans-Martin; Ammelburg, Moritz; Cerdà, Joan ; Nüsslein-Volhard, Christiane; Irion, Uwe
Palabras claveAquaporin
Pattern formation
Pigmentation
Zebrafish
Fecha de publicaciónjun-2017
EditorCompany of Biologists
CitaciónDevelopment 144(11): 2059-2069 (2017)
ResumenThe development of the pigmentation pattern in zebrafish is a tightly regulated process that depends on both the self-organizing properties of pigment cells and extrinsic cues from other tissues. Many of the known mutations that alter the pattern act cell-autonomously in pigment cells, and our knowledge about external regulators is limited. Here, we describe novel zebrafish mau mutants, which encompass several dominant missense mutations in Aquaporin 3a (Aqp3a) that lead to broken stripes and short fins. A loss-of-function aqp3a allele, generated by CRISPR-Cas9, has no phenotypic consequences, demonstrating that Aqp3a is dispensable for normal development. Strikingly, the pigment cells from dominant mau mutants are capable of forming a wild-type pattern when developing in a wild-type environment, but the surrounding tissues in the mutants influence pigment cell behaviour and interfere with the patterning process. The mutated amino acid residues in the dominant alleles line the pore surface of Aqp3a and influence pore permeability. These results demonstrate an important effect of the tissue environment on pigment cell behaviour and, thereby, on pattern formation
Descripción11 pages, 6 figures, 1 table, supplementary information https://dx.doi.org/10.1242/dev.143495
Versión del editorhttps://dx.doi.org/10.1242/dev.143495
URIhttp://hdl.handle.net/10261/153274
DOI10.1242/dev.143495
Identificadoresdoi: 10.1242/dev.143495
issn: 0950-1991
e-issn: 1477-9129
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