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Detection of carvacrol in biological tissues by analytical pyrolysis

AuthorsCameán Fernández, A. M.; Llana Ruiz-Cabello, M.; Puerto Rodríguez, María; Pichardo, S.; Jiménez Morillo, N. T. ; González-Pérez, José Antonio
Issue DateSep-2016
CitationToxicology Letters 258 ( Supplement): S173-S174 (2016)
AbstractOregano essential oil is being included in new packaging materials due to its bioactive properties. Carvacrol, main compound of this essential oil, with antioxidant and antimicrobial properties, is intended to be used in food packaging. According to the recommendation of the EFSA for the genotoxic assessment of substances in food, the in vivo negative results are acceptable when direct or indirect evidence supportive of exposure of the target tissues have been demonstrated. Previous studies reported negative results for comet assay in stomach and liver of rats orally exposed to carvacrol (81, 256 and 810 mg/kg bw). Hence, in this work, direct pyrolysis–gas chromatography–mass spectrometry (Py–GC/MS) analysis was performed using a double-shot pyrolyzer attached to a GC/MS system to ensure the exposure of such tissues to carvacrol. Results showed the presence of carvacrol in stomach and liver in all of the concentrations assayed. Moreover, pyrolysis technique showed clear and distinct dose–response relationships depending on the tissue evaluated. However, the ingestion of concentrations over 256 mg/kg bw in the liver, exhibited no-linear relation. This does not relate to the ratios predicted by the linear model found in the stomach. In this regard, the differences observed in those curves may be related to carvacrol metabolism in rats and the possible occurrence of saturation mechanisms limiting an excess of carvacrol metabolization/presence in liver. Therefore, our results indicate that Py–GC/MS is a valuable tool to evaluate the exposure of biological tissues to carvacrol avoiding sample pre-treatmen
DescriptionAbstracts of the 52nd Congress of the European Societies of Toxicology (EUROTOX) Fibes Congress Center Seville, Spain, 04th-07th September 2016
Publisher version (URL)https://doi.org/10.1016/j.toxlet.2016.06.1649
Appears in Collections:(IRNAS) Artículos
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