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The role of PPARγ in endoplasmic reticulum (ER) stress in podocyte injury induced by palmitic acid

AutorIzquierdo Lahuerta, Adriana; Corrales-Cordón, Patricia; Valverde, Ángela M.; Coward, R. J.; Medina, Gema
Fecha de publicación2015
CitaciónXXXVIII Congreso de la SEBBM (2015)
ResumenChanges in lifestyle and dietary habits have raised the overall incidence of obesity. Obese patients often suffer other comorbidities such as hypertension, heart disease, dyslipidaemia, type 2 diabetes and renal disease. Recently, evidences suggest that renal lipid accumulation leads to glomerular damage and more specifi cally, whether this accumulation produces podocyte dysfunction. An imbalance between protein load and folding capacity is referred to as endoplasmic reticulum (ER) stress. As a defense mechanism, cells express ER stress inducible chaperons, as glucose-regulated proteins (GRPs).The aim of this study was to analyze the role of PPARγ in the maintenance of the integrity of the podocyte and the involvement of ER stress in podocyte injury induced by palmitic acid (PA) and/or High Glucose (HG). We have used two lines of mouse podocytes (WT and PPARγ knockdown) treated with PA (500μM) and/or HG(25mM) with/without the thiazolidinedione Pioglitazone (Pio) (0.1 μm) for 24h. PA treatment produced oxidative stress and endoplasmic reticulum stress by IRE1 pathway with 10- fold increase in gene expression of GRP78, sXBP1and CHOP. Furthermore rearrangements of the actin cytoskeleton (CD2AP) by PA was observed in the WT podocyte, but these effects were signifi cant greater in podocytes PPARγ knockdown. Pio prevented PA-induced injury in WT podocyte, but this injury did not ameliorated in podocytes PPARγ knockdown. This study suggests a crucial role PPARγ in the protection against ER stress in the podocyte injury induced by Palmitic acid.
DescripciónResumen del póster presentado al XXXVIII Congreso de la Sociedad Española de Bioquímica y Biología Molecular, celebrado en Valencia del 7 al 10 de septiembre de 2015.-- et al.
URIhttp://hdl.handle.net/10261/153223
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