English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/152934
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

A Novel Typing Method for Streptococcus pneumoniae Using Selected Surface Proteins

AuthorsDomenech, Arnau; Moreno, Javier ; Ardanuy, Carmen; Liñares, Josefina; Campa, Adela G. de la; Martin Galiano, Antonio J.
KeywordsSurface proteins
Emergent clones
Genomics
Diagnosis
Virulence factors
Issue Date31-Mar-2016
PublisherFrontiers Media
CitationFrontiers in Microbiology 7: 420 (2016)
AbstractThe diverse pneumococcal diseases are associated with different pneumococcal lineages, or clonal complexes. Nevertheless, intra-clonal genomic variability, which influences pathogenicity, has been reported for surface virulence factors. These factors constitute the communication interface between the pathogen and its host and their corresponding genes are subjected to strong selective pressures affecting functionality and immunogenicity. First, the presence and allelic dispersion of 97 outer protein families were screened in 19 complete pneumococcal genomes. Seventeen families were deemed variable and were then examined in 216 draft genomes. This procedure allowed the generation of binary vectors with 17 positions and the classification of strains into surfotypes. They represent the outer protein subsets with the highest inter-strain discriminative power. A total of 116 non-redundant surfotypes were identified. Those sharing a critical number of common protein features were hierarchically clustered into 18 surfogroups. Most clonal complexes with comparable epidemiological characteristics belonged to the same or similar surfogroups. However, the very large CC156 clonal complex was dispersed over several surfogroups. In order to establish a relationship between surfogroup and pathogenicity, the surfotypes of 95 clinical isolates with different serogroup/serotype combinations were analyzed. We found a significant correlation between surfogroup and type of pathogenic behavior (primary invasive, opportunistic invasive, and non-invasive). We conclude that the virulent behavior of S. pneumoniae is related to the activity of collections of, rather than individual, surface virulence factors. Since surfotypes evolve faster than MLSTs and directly reflect virulence potential, this novel typing protocol is appropriate for the identification of emerging clones.
Publisher version (URL)http://dx.doi.org/10.3389/fmicb.2016.00420
URIhttp://hdl.handle.net/10261/152934
DOI10.3389/fmicb.2016.00420
ISSN1664-302X
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
Novel_Typing_Method for_Streptococcus_pneumoniae.pdf2,32 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.