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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/15276

Analysis of bisphenol A in natural waters by means of an optical immunosensor

AutorRodríguez-Mozaz, Sara; López de Alda, Miren; Barceló, Damià
Palabras claveImmunosensor
Bisphenol A
Fecha de publicación6-dic-2005
CitaciónWater Research 39(20): 5071-5079 (2005)
ResumenThis work describes a very simple, fast and sensitive method based on the use of the optical immunosensor “RIver ANAlyser” (RIANA) to the determination of bisphenol A in a waters. RIANA is based on a rapid solid-phase indirect inhibition immunoassay that takes place at an optical transducer chip chemically modified with an analyte derivative. Fluorescence produced by labelled antibodies bound to the transducer is detected by photodiodes and can be correlated with the analyte concentration. The sensor surface can be regenerated thus allowing the performance of several measurements (around 300) with the same transducer. Each test cycle, including one regeneration step, is accomplished in 15 min. The detection limit achieved in the direct determination of bisphenol A in water with this system was 0.014 μg/L. Satisfactory repeatability, with relative standard deviations (RSD) ranging between 1.48% and 6.93% were obtained. The immunosensor method developed was applied to the monitoring of bisphenol A in various types of water collected in a waterworks (from the river water source to the finished drinking water) and validated against the results obtained in the same approach by a more traditional method, based on solid-phase extraction followed by liquid chromatography–mass spectrometry. Results obtained by both techniques were in general good agreement (considering the typical overestimation bias of immunoassays), and served to prove the satisfactory removal efficiency of the overall purification process applied in the waterworks and, in particular, of the sand filtration step.
Descripción9 pages, 3 figures.-- PMID: 16337256 [PubMed].-- Printed version published Dec 2005.
Versión del editorhttp://dx.doi.org/10.1016/j.watres.2005.09.023
ISSN0043-1354 (Print)
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