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N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation

AuthorsSoto, Daniela; Pieralisi, Azul; Gómez-Serrano, María; Peral, Belén ; Calvo, Juan C.; Guerra, Liliana N.
Issue Date2015
CitationOCC World Congress (2015)
AbstractAntioxidants in obesity have provided contradictory results. We used 3T3-L1 preadipocytes (undifferentiated cells: CC), which are capable of differentiating into mature adipocytes (differentiated cells: DC), which accumulate triglycerides (Tg) and express proteins related to oxidative stress such as monoamine oxidase A (MAOA). Following the differentiation of preadipocytes, an increase in the levels of phospho-kinases (AKT, JNK and ERK½) is observed, with mitotic cellular expansion (MCE) in the first days of cellular differentiation. We have demonstrated that N-acetylcysteine (NAC) inhibited Tg accumulation in DC (Redox Reports, 2013). Here we determined that 10 μM NAC treatment did not affect AKT phosphorylation, but significantly inhibited JNK phosphorylation (DC: 10.02 ± 0.45 vs DC 10 μM NAC: 5.53 ± 1.04 arbitrary units (AU), p< 0.01); higher doses of NAC (up to 1mM) provoked similar inhibition. 10 μM or 1mM NAC provoked 80% decrease on ERK½ phosphorylation, evaluating pERK½/ERK (DC: 2.24 ± 0.50 vs DC 10μM NAC: 0.40 ± 0.07 AU; p< 0.01). At day 2 of adipogenic differentiation, MCE was observed in DC (DC: 1.3x105 ± 0.18x105 cells/ mL vs CC: 0.51x105 ± 0.09x105 cells/ mL, p<0.01), NAC significantly inhibited MCE (DC vs DC 10 μM NAC: 0.49x105 cells/ mL ± 0.03x105 cells/ mL, p<0.01; DC vs DC 1mM NAC: 0.65x105 cells/mL ± 0.04x105 cells/ mL, p<0.01). NAC significantly decreased mitochondrial MAOA expression in a dose dependent manner (DC: 1.00 ± 0.84 vs DC 10 μM NAC: 0.70 ± 0.03 AU, p< 0.01; DC vs DC 1mM NAC: 0.34 ± 0.01 AU p<0.01). We postulated that NAC could inhibit lipid accumulation and mitochondrial protein expression by inhibiting both mitotic cellular expansion and kinase phosphorylation as a part of a MAPK pathway during adipogenic differentiation.
DescriptionResumen del póster presentado al Oxygen Club of California (OCC) World Congress: "Oxidants and antioxidants in biology", celebrado en Valencia (España) del 24 al 26 de junio de 2015.
Appears in Collections:(IIBM) Comunicaciones congresos
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