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i-motif structures in long cytosine-rich sequences found upstream of the promoter region of the SMARCA4 gene

AutorBenabou, Sanae; Aviñó, Anna; Lyonnais, Sébastien; Bustamante González, Carlos Alberto; Eritja Casadellà, Ramón; De Juan, Anna; Gargallo, Raimundo
Palabras claveCrowding
Fecha de publicaciónsep-2017
CitaciónBiochimie 140: 20-33 (2017)
ResumenCytosine-rich oligonucleotides are capable of forming complex structures known as i-motif with increasingly studied biological properties. The study of sequences prone to form i-motifs located near the promoter region of genes may be difficult because these sequences not only contain repeats of cytosine tracts of disparate length but also these may be separated by loops of varied nature and length. In this work, the formation of intramolecular i-motif structures by a long sequence located upstream of the promoter region of the SMARCA4 gene has been demonstrated. Nuclear Magnetic Resonance, Circular Dichroism, Gel Electrophoresis, Size-Exclusion Chromatography, and multivariate analysis have been used. Not only the wild sequence (5‘-TC3T2GCTATC3TGTC2TGC2TCGC3T2G2TCATGA2C4-3’) has been studied but also several other truncated and mutated sequences. Despite the apparent complex sequence, the results showed that the wild sequence may form a relatively stable and homogeneous unimolecular i-motif structure, both in terms of pH or temperature. The model ligand TMPyP4 destabilizes the structure, whereas the presence of 20% (w/v) PEG200 stabilized it slightly. This finding opens the door to the study of the interaction of these kind of i-motif structures with stabilizing ligands or proteins. © 2017 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM)
Versión del editorhttp://doi.org/10.1016/j.biochi.2017.06.005
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