English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/152476
Título

Initial expression of phosphoenolpyruvate carboxykinase gene in fetal hepatocytes: Role of transcription factors

AutorMolero, Cristina; Valverde, Ángela M.; Teruel, Teresa; Benito, Manuel; Lorenzo, Margarita
Palabras clavemRNA level
Phorbol esters
Transcription rate
PEPCK
Insulin
C/EBPs
c-fos
c-myc
Dibutyryl cAMP
Fetal hepatocytes
Fecha de publicación1996
EditorElsevier
CitaciónJournal of Hepatology 25(4): 510-517 (1996)
Resumen[Background/Aims]: Hepatic phosphoenolpyruvate carboxykinase (PEPCK; EC 4.1.1.32) gene is absent in fetal liver. However, this gene can be initially expressed in 20-day-old fetal hepatocyte primary cultures under specific hormonal stimulation. The role of transcriptional factors involved is also studied. [Methods]: Primary 20-day-old fetal hepatocytes have been cultured and Northern-blot and nuclear run-on transcription assays have been performed. [Results]: Fetal hepatocytes in culture initially expressed PEPCK gene by dibutyryl cAMP, in the presence of dexamethasone. Dibutyryl cAMP increased by 8-fold the rate of transcription of PEPCK gene at 30 min, and produced a 50-fold increase in its mRNA content at 3 h. This induction of PEPCK expression by cAMP occurred in the presence of sustained levels of CCAAT/enhancer binding protein (C/EBP)α-δ mRNAs, and was accompanied by an increase in the rate of transcription and mRNA content of C/EBPβ gene, and a decrease in the expression of c-myc, in the absence of c-fos expression. In addition, insulin or phorbol esters decreased by 50% the PEPCK rate of transcription and its mRNA accumulation induced by dibutyryl cAMP This inhibitory effect of insulin or phorbol esters on PEPCK gene expression was accompanied by an increase in the rate of transcription and mRNA content of nuclear factors such as c-fos and c-myc, the expression of C/EBPs remaining essentially unmodified.
URIhttp://hdl.handle.net/10261/152476
DOI10.1016/S0168-8278(96)80211-X
Identificadoresdoi: 10.1016/S0168-8278(96)80211-X
issn: 0168-8278
e-issn: 1600-0641
Aparece en las colecciones: (IIBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.