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http://hdl.handle.net/10261/152474
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Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Teruel, Teresa | - |
dc.contributor.author | Valverde, Ángela M. | - |
dc.contributor.author | Benito, Manuel | - |
dc.contributor.author | Lorenzo, Margarita | - |
dc.date.accessioned | 2017-07-07T09:25:21Z | - |
dc.date.available | 2017-07-07T09:25:21Z | - |
dc.date.issued | 1996 | - |
dc.identifier | doi: 10.1002/(SICI)1097-4652(199603)166:3<577::AID-JCP12>3.0.CO;2-4 | - |
dc.identifier | issn: 0021-9541 | - |
dc.identifier | e-issn: 1097-4652 | - |
dc.identifier.citation | Journal of Cellular Physiology 166(3): 577-584 (1996) | - |
dc.identifier.uri | http://hdl.handle.net/10261/152474 | - |
dc.description.abstract | The presence of transforming growth factor β1 (TGF-β1) for 24 or 48 h stimulated DNA synthesis, the percentage of cells in the S + G2/M phases of the cell cycle, and cell number, as compared to quiescent cells. The mitogenic capacity of TGF-β1 (1 pM) was similar to that shown by 10% fetal calf serum (FCS). TGF-β1 for 48 h increased by 5-fold the percentage of cells containing (3H)thymidine-labeled nuclei as compared to quiescent cells. In addition, single fetal brown adipocytes, showing their typical multilocular fat droplets phenotype, become positive for (3H)thymidme-labeled nuclei in response to TGF-β1. Moreover, TGF-β1 induced the mRNA expression of a complete set of proliferation-related genes, such as c-fos (30 min), c-myc and β-actin (2 h), and H-ras, cdc2 kinase, and glucose 6-phosphate dehydrogenase (G6PD) at 4 and 8 h, as compared to quiescent cells. Concurrently, TGF-β1 for 12 h increased the protein content of proliferating cellular nuclear antigen (PCNA) by 6-fold and p21-ras by 2-fold. Although our results demonstrate that TGF-β1 induces the expression of very early genes related to cell proliferation, TGF-β1 could be acting either as a mitogen or as a survival factor to induce proliferation in fetal brown adipocytes. | - |
dc.description.sponsorship | Funded by: Comisión Interministorial de Ciencia y Technologia, Spain. Grant Number: SAF 93/0206. | - |
dc.publisher | Wiley-Liss | - |
dc.rights | closedAccess | - |
dc.title | Transforming growth factor β1 induces mitogenesis in fetal rat brown adipocytes | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1002/(SICI)1097-4652(199603)166:3<577::AID-JCP12>3.0.CO;2-4 | - |
dc.date.updated | 2017-07-07T09:25:21Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | Comisión Interministerial de Ciencia y Tecnología, CICYT (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100007273 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
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