English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/152428
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


The addiction-related protein ANKK1 is differentially expressed during the cell cycle in neural precursors

AuthorsEspaña-Serrano, Laura; Guerra Martín-Palacio, Noelia; Montero-Pedrazuela, Ana ; Pérez-Santamarina, Estela; Vidal, Rebeca ; García-Consuegra, Inés; Valdizán, Elsa M. ; Pazos, Ángel ; Palomo, Tomás; Jiménez-Arriero, Miguel Ángel; Guadaño-Ferraz, Ana ; Hoenicka, Janet
Cell cycle
Issue Date2017
PublisherOxford University Press
CitationCerebral Cortex 27(5): 2809-2819 (2017)
AbstractTaqIA is a polymorphismassociated with addictions and dopamine-related traits. It is located in the ankyrin repeat and kinase domain containing 1 gene (ANKK1) nearby the gene for the dopamine D2 receptor (D2R). Since ANKK1 function is unknown, TaqIA-associated traits have been explained only by differences in D2R. Here we report ANKK1 studies in mouse and human brain using quantitative real-time PCR, Western blot, immunohistochemistry, and flow cytometry. ANKK1 mRNA and protein isoforms vary along neurodevelopment in the human and mouse brain. In mouse adult brain ANKK1 is located in astrocytes, nuclei of postmitotic neurons and neural precursors from neurogenic niches. In both embryos and adults, nuclei of neural precursors showsignificant variation of ANKK1 intensity.We demonstrate a correlation between ANKK1 and the cell cycle. Cell synchronization experiments showed a significant increment of ANKK1-kinase in mitotic cells while ANKK1-kinase overexpression affects G1 and M phase that were found to be modulated by ANKK1 alleles and apomorphine treatment. Furthermore, during embryonic neurogenesis ANKK1 was expressed in slow-dividing neuroblasts and rapidly dividing precursors which are mitotic cells. These results suggest a role of ANKK1 during the cell cycle in neural precursors thus providing biological support to brain structure involvement in the TaqIA-associated phenotypes.
Identifiersdoi: 10.1093/cercor/bhw129
e-issn: 1460-2199
issn: 1047-3211
Appears in Collections:(IBBTEC) Artículos
(IIBM) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.