English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/152313
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Nitric oxide regulates mitochondrial oxidative stress protection via the transcriptional coactivator PGC-1α

AuthorsBorniquel, Sara; Valle, Inmaculada; Cadenas, Susana; Lamas Peláez, Santiago ; Monsalve, María
Issue Date2006
PublisherFederation of American Societies for Experimental Biology
CitationFASEB Journal 20(11): 1889-1891 (2006)
AbstractNitric oxide (NO) has both prooxidant and antioxidant activities in the endothelium; however, the molecular mechanisms involved are still a matter of controversy. PGC-1α [peroxisome proliferators-activated receptor (PPAR) γ coactivator 1-α] induces the expression of several members of the mitochondrial reactive oxygen species (ROS) detoxification system. Here, we show that NO regulates this system through the modulation of PGC-1α expression. Short-term (<12 h) treatment of endothelial cells with NO donors down-regulates PGC-1α expression, whereas long-term (>24 h) treatment up-regulates it. Treatment with the NOS inhibitor L-NAME has the opposite effect. Downregulation of PGC-1α by NO is mediated by protein kinase G (PKG). It is blocked by the soluble guanylate cyclase (sGC) inhibitor ODQ and the PKG inhibitor KT5823, and mimicked by the cGMP analog 8-Br-cGMP. Changes in PGC-1α expression are in all cases paralleled by corresponding variations in the mitochondrial ROS detoxification system. Cells that transiently overexpress PGC-1α from the cytomeglovirus (CMV) promoter respond poorly to NO donors. Analysis of tissues from eNOS-/- mice showed reduced levels of PGC-1α and the mitochondrial ROS detoxification system. These data suggest that NO can regulate the mitochondrial ROS detoxification system both positively and negatively through PGC-1α.
Identifiersdoi: 10.1096/fj.05-5189fje
issn: 0892-6638
e-issn: 1530-6860
Appears in Collections:(IIBM) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.