Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/152112
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

Short-term grafting of human neural stem cells: Electrophysiological properties and motor behavioral amelioration in experimental Parkinson’s disease

AuthorsMartínez-Serrano, Alberto CSIC ORCID; Pereira, Marta P. CSIC ORCID; Avaliani, Natalia; Nelke, Nelke; Kokaia, Merab; Ramos-Moreno, Tania CSIC ORCID
KeywordsVentral mesencephalon
Electrophysiology
A9-dopaminergic phenotype
Human neural stem cells (hNSCs)
Parkinson’s disease (PD)
Issue Date17-Jun-2016
PublisherCognizant Communication Corporation
CitationCell Transplantation 25: 2083- 2097 (2016)
AbstractCell replacement therapy in Parkinson’s disease (PD) still lacks a study addressing the acquisition of electrophysiological properties of human grafted neural stem cells and their relation with the emergence of behavioral recovery after transplantation in the short term. Here we study the electrophysiological and biochemical profiles of two ventral mesencephalic human neural stem cell (NSC) clonal lines (C30-Bcl-X and C32-Bcl-X) that express high levels of Bcl-X to enhance their neurogenic capacity, after grafting in an in vitro parkinsonian model. Electrophysiological recordings show that the majority of the cells derived from the transplants are not mature at 6 weeks after grafting, but 6.7% of the studied cells showed mature electrophysiological profiles. Nevertheless, parallel in vivo behavioral studies showed a significant motor improvement at 7 weeks postgrafting in the animals receiving C30-Bcl-X, the cell line producing the highest amount of TH cells. Present results show that, at this postgrafting time point, behavioral amelioration highly correlates with the spatial dispersion of the TH grafted cells in the caudate putamen. The spatial dispersion, along with a high number of dopaminergic-derived cells, is crucial for behavioral improvements. Our findings have implications for long-term standardization of stem cell-based approaches in Parkinson’s disease.
URIhttp://hdl.handle.net/10261/152112
DOI10.3727/096368916X692069
Identifiersdoi: 10.3727/096368916X692069
issn: 0963-6897
Appears in Collections:(CBM) Artículos

Files in This Item:
File Description SizeFormat
Martínez-Serrano_Short-TermGraftingofHuman.pdf2,28 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work

WEB OF SCIENCETM
Citations

1
checked on Nov 23, 2021

Google ScholarTM

Check

Altmetric

Dimensions


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.