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Título

Role of proteolipid protein in HSV-1 entry in oligodendrocytic cells

AutorBello-Morales, Raquel; Crespillo, Antonio Jesús ; Praena, Beatriz; Tabarés, Enrique; Revilla Novella, Yolanda ; García, Elena; Fraile-Ramos, Alberto; Baron, Wia; Krummenacher, Claude; López-Guerrero, José Antonio
Fecha de publicación25-ene-2016
EditorPublic Library of Science
CitaciónPLoS ONE 11 (2016)
ResumenHerpes simplex virus type 1 (HSV-1) has the ability to enter many different hosts and cell types by several strategies. This highly prevalent alphaherpesvirus can enter target cells using different receptors and different pathways: fusion at a neutral pH, low-pH-dependent and low-pH-independent endocytosis. Several cell receptors for viral entry have been described, but several observations suggest that more receptors for HSV-1 might exist. In this work, we propose a novel role for the proteolipid protein (PLP) in HSV-1 entry into the human oligodendrocytic cell line HOG. Cells transfected with PLP-EGFP showed an increase in susceptibility to HSV-1. Furthermore, the infection of HOG and HOG-PLP transfected cells with the R120vGF virus-unable to replicate in ICP4-defficient cells-showed an increase in viral signal in HOG-PLP, suggesting a PLP involvement in viral entry. In addition, a mouse monoclonal antibody against PLP drastically inhibited HSV-1 entry into HOG cells. PLP and virions colocalized in confocal immunofluorescence images, and in electron microscopy images, which suggest that PLP acts at the site of entry into HOG cells. Taken together these results suggest that PLP may be involved in HSV-1 entry in human oligodendrocytic cells.
URIhttp://hdl.handle.net/10261/152099
DOI10.1371/journal.pone.0147885
Identificadoresdoi: 10.1371/journal.pone.0147885
issn: 1932-6203
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