Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/152091
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Role of bifidobacteria in the hydrolysis of chlorogenic acid |
Autor: | Raimondi, Stefano; Anighoro, Andrew; Quartieri, Andrea; Amaretti, Alberto; Tomás Barberán, Francisco CSIC ORCID ; Rastelli, Giulio; Rossi, Maddalena | Palabras clave: | Bifidobacteria Bifidobacterium animalis Caffeic acid Chlorogenic acids Feruloyl esterase Hydroxycinnamic acids Probiotics |
Fecha de publicación: | feb-2015 | Editor: | John Wiley & Sons | Citación: | MicrobiologyOpen 4(1): 41-52 (2015) | Resumen: | This study aimed to explore the capability of potentially probiotic bifidobacteria to hydrolyze chlorogenic acid into caffeic acid (CA), and to recognize the enzymes involved in this reaction. Bifidobacterium strains belonging to eight species occurring in the human gut were screened. The hydrolysis seemed peculiar of Bifidobacterium animalis, whereas the other species failed to release CA. Intracellular feruloyl esterase activity capable of hydrolyzing chlorogenic acid was detected only in B. animalis. In silico research among bifidobacteria esterases identified Balat_0669 as the cytosolic enzyme likely responsible of CA release in B. animalis. Comparative modeling of Balat_0669 and molecular docking studies support its role in chlorogenic acid hydrolysis. Expression, purification, and functional characterization of Balat_0669 in Escherichia coli were obtained as further validation. A possible role of B. animalis in the activation of hydroxycinnamic acids was demonstrated and new perspectives were opened in the development of new probiotics, specifically selected for the enhanced bioconversion of phytochemicals into bioactive compounds. | Versión del editor: | http://doi.org/10.1002/mbo3.219 | URI: | http://hdl.handle.net/10261/152091 | DOI: | 10.1002/mbo3.219 | ISSN: | 2045-8827 |
Aparece en las colecciones: | (CEBAS) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Raimondi_et_al-2015-MicrobiologyOpen.pdf | 1,21 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
16
checked on 16-abr-2024
SCOPUSTM
Citations
50
checked on 16-abr-2024
WEB OF SCIENCETM
Citations
46
checked on 24-feb-2024
Page view(s)
254
checked on 19-abr-2024
Download(s)
275
checked on 19-abr-2024