English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/152065
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

RhoB controls endothelial barrier recovery by inhibiting Rac1 trafficking to the cell border

AuthorsMarcos Ramiro, Beatriz ; García-Weber, Diego ; Barroso, Susana ; Ortega, María C.; Reglero-Real, Natalia ; Fernández Martín, Laura ; Alonso, Miguel A. ; Correas, Isabel ; Millán, Jaime
Issue Date2-May-2016
CitationJournal of Cell Biology 213: 385- 402 (2016)
AbstractEndothelial barrier dysfunction underlies chronic inflammatory diseases. In searching for new proteins essential to the human endothelial inflammatory response, we have found that the endosomal GTPase RhoB is up-regulated in response to inflammatory cytokines and expressed in the endothelium of some chronically inflamed tissues. We show that although RhoB and the related RhoA and RhoC play additive and redundant roles in various aspects of endothelial barrier function, RhoB specifically inhibits barrier restoration after acute cell contraction by preventing plasma membrane extension. During barrier restoration, RhoB trafficking is induced between vesicles containing RhoB nanoclusters and plasma membrane protrusions. The Rho GTPase Rac1 controls membrane spreading and stabilizes endothelial barriers. We show that RhoB colocalizes with Rac1 in endosomes and inhibits Rac1 activity and trafficking to the cell border during barrier recovery. Inhibition of endosomal trafficking impairs barrier reformation, whereas induction of Rac1 translocation to the plasma membrane accelerates it. Therefore, RhoB-specific regulation of Rac1 trafficking controls endothelial barrier integrity during inflammation
URIhttp://hdl.handle.net/10261/152065
DOI10.1083/jcb.201504038
Identifiersdoi: 10.1083/jcb.201504038
issn: 1540-8140
Appears in Collections:(CBM) Artículos
Files in This Item:
File Description SizeFormat 
MillánJ_RhoBControlEndothelialBarrier.pdf5,07 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.