English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/152031
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Green synthesis of β-sitostanol esters catalyzed by the versatile lipase/sterol esterase from Ophiostoma piceae

AuthorsMolina-Gutiérrez, María; Hakalin, Neumara L. S.; Rodríguez-Sánchez, Leonor ; Prieto, Alicia ; Martínez, María Jesús
KeywordsEnzymatic esterification
Solvent-free transesterification
Sitostanyl ester
Phytosterols
Lipase
Sterol esterase
Issue Date15-Apr-2017
PublisherElsevier
CitationFood Chemistry 221:1458–1465 (2017)
Abstractβ-sitostanol esters, used as dietary complement for decreasing cholesterol absorption, have been synthesized at 28 °C via direct esterification or transesterification catalyzed by the versatile lipase/sterol esterase from the ascomycete fungus O. piceae. Direct esterification was conducted in biphasic isooctane: water systems containing 10 mM β-sitostanol and lauric or oleic acid as acyl donors, reaching 90% esterification in 3 h with the recombinant enzyme. The use of molar excesses of the free fatty acids did not improve direct esterification rate, and the enzyme did not convert one of the two fatty acids preferentially when both were simultaneously available. On the other hand, solvent-free transesterification was an extremely efficient mechanism to synthesize β-sitostanyl oleate, yielding virtually full conversion of up to 80 mM β-sitostanol in 2 h. This process may represent a promising green alternative to the current chemical synthesis of these esters of unquestionable nutraceutical value.
Description24 p.-6 fig.
Publisher version (URL)https://doi.org/10.1016/j.foodchem.2016.11.005
URIhttp://hdl.handle.net/10261/152031
DOI10.1016/j.foodchem.2016.11.005
E-ISSN0308-8146
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
Postprint Molina-Gutiérrez et al 2017.docxPostprint793,86 kBMicrosoft Word XMLView/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.