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Title

Characterization of 12 polymorphic microsatellite markers in the sugar kelp Saccharina latissima

AuthorsPaulino, Cristina M.; Neiva, João; Coelho, Nelson C.; Aires, Tánia; Marbà, Núria CSIC ORCID ; Krause-Jensen, Dorte; Serrao, Ester Álvares
KeywordsGenetic differentiation
Phaeophyceae
Saccharina latissima
Microsatellite markers
Kelp
Gene diversity
Issue DateOct-2016
PublisherSpringer
CitationJournal of Applied Phycology 28: 3071-3074 (2016)
AbstractSaccharina latissima is an ecologically and economically important kelp species native to the coastal regions of the Northern Hemisphere. This species has considerable phylogeographic structure and morphological plasticity, but lack of resolution of available genetic markers prevents a finer characterization of its genetic diversity and structure. Here, we describe 12 microsatellite loci identified in silico in a genomic library, and assess their polymorphism in three distant populations. Allelic richness at the species level was relatively high (5–23 alleles per locus), as was gene diversity within populations (0.42 < H < 0.62). In addition, individuals readily form distinct genotypic clusters matching their populations of origin. The variation detected confirms the great potential of these markers to investigate the biogeography and population dynamics of S. latissima, and to better characterize its genetic resources for the establishing farming industry.
Publisher version (URL)https://doi.org/10.1007/s10811-016-0811-x
URIhttp://hdl.handle.net/10261/151793
DOIhttp://dx.doi.org/10.1007/s10811-016-0811-x
Identifiersdoi: 10.1007/s10811-016-0811-x
issn: 1573-5176
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