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Plasmidic vectors for characterization of gene expression in, and fluorescent labeling of, Gram-positive bacteria

AuthorsRuiz-Masó, José A. ; García-Cayuela, Tomás ; Fernández de Palencia, P. ; Solar, Gloria del ; Mohedano Bonillo, Mari Luz
Issue Date2016
CitationBioIberoAmérica 2016
AbstractVectors based on plasmids have been constructed and widely used in Gram-negative bacteria for a variety of purposes. However, these kinds of genetic tools are still scarce in most Gram-positive bacteria. We have developed various plasmid vectors that permit: i) the expression of genes, ii) the detection and quantification of gene expression in Gram-positive bacteria, and iii) the use of labelled bacteria in in vitro models (interactions between bacteria and eukaryotic cells) and also in in vivo models (bacterial colonization of the digestive tract of zebrafish). These vectors encode versions of the fluorescence proteins: green (GFP) from Aqueora Victoria and red (a monomeric version, mCherry) from Dicosoma sp., whose coding genes have been optimized for expression in bacteria. Importantly, the spectra of excitation and emission of both proteins allow their simultaneous detection. The vectors function in (among others) Lactococcus lactis, Streptococcus pneumoniae, Staphylococcus aureus, Listeria monocytogenes and Pediococcus parvulus, as well as in various species belonging to the genera Lactobacillus and Enterococcus, and are being commercialized by the company Solmeglas http://solmeglas.com/es/118-vectores-de-expresion-genica.
DescriptionResumen del trabajo presentado al 1er Congreso Iberoamericano de Biotecnología, celebrado en Salamanca (España) del 5 al 8 de junio de 2016.
Appears in Collections:(CIAL) Comunicaciones congresos
(CIB) Comunicaciones congresos
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