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Título

A simple and efficient method to prepare pure dimers and monomers of the cytochrome b6f complex from spinach

AutorLuján Serrano, María Ángeles ; Lorente Lorente, Patricia; Zazubovich, Valter; Picorel Castaño, Rafael
Palabras claveCytochrome b6f
Photosynthesis
Plant
Protein
Purification
SDS-PAGE
Fecha de publicaciónjun-2017
EditorSpringer
CitaciónLuján MA, Lorente P, Zazubovich V, Picorel R. A simple and efficient method to prepare pure dimers and monomers of the cytochrome b6f complex from spinach. Photosynthesis Research 132 (3): 305-309 (2017)
ResumenUsing a single size-exclusion chromatography we were able to isolate highly pure dimers and monomers of the Cyt b6f complex from spinach from a bulk preparation of that protein complex obtained with a standard procedure. At higher protein/detergent ratio during the chromatography most of the Cyt b6f complex remained as dimers. In contrast, at lower protein/detergent ratio (around 15 times lower), most dimers became monomerized. As a bonus, this chromatography also allowed the elimination of potential Chl a contaminant to the Cyt b6f preparations. SDS-PAGE protein analysis with 18% (w/v) acrylamide revealed the loss of the ISP subunit in our monomeric preparation. However, it fully retained the content of Chl a, a prerequisite to perform any spectroscopic study involving this unique pigment.
Descripción16 Pags.- 4 Figs. The definitive version is available at: https://link.springer.com/journal/11120
Versión del editorhttp://dx.doi.org/10.1007/s11120-017-0375-x
URIhttp://hdl.handle.net/10261/151480
DOI10.1007/s11120-017-0375-x
ISSN0166-8595
E-ISSN1573-5079
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