English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/151389
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Evaluating intra- and inter-individual variation in the human placental transcriptome

AutorHughes, David A.; Kircher, Martin; He, Zhisong; Guo, Song; Fairbrother, Genevieve L.; Moreno, Carlos S.; Khaitovich, Philipp; Stoneking, Mark
Fecha de publicación19-mar-2015
EditorBioMed Central
CitaciónGenome Biology 16: 54 (2015)
Resumen[Background] Gene expression variation is a phenotypic trait of particular interest as it represents the initial link between genotype and other phenotypes. Analyzing how such variation apportions among and within groups allows for the evaluation of how genetic and environmental factors influence such traits. It also provides opportunities to identify genes and pathways that may have been influenced by non-neutral processes. Here we use a population genetics framework and next generation sequencing to evaluate how gene expression variation is apportioned among four human groups in a natural biological tissue, the placenta.
[Results] We estimate that on average, 33.2%, 58.9%, and 7.8% of the placental transcriptome is explained by variation within individuals, among individuals, and among human groups, respectively. Additionally, when technical and biological traits are included in models of gene expression they each account for roughly 2% of total gene expression variation. Notably, the variation that is significantly different among groups is enriched in biological pathways associated with immune response, cell signaling, and metabolism. Many biological traits demonstrate correlated changes in expression in numerous pathways of potential interest to clinicians and evolutionary biologists. Finally, we estimate that the majority of the human placental transcriptome exhibits expression profiles consistent with neutrality; the remainder are consistent with stabilizing selection, directional selection, or diversifying selection.
[Conclusions] We apportion placental gene expression variation into individual, population, and biological trait factors and identify how each influence the transcriptome. Additionally, we advance methods to associate expression profiles with different forms of selection.
Versión del editorhttp://dx.doi.org/10.1186/s13059-015-0627-z
URIhttp://hdl.handle.net/10261/151389
DOI10.1186/s13059-015-0627-z
ISSN1474-760X
E-ISSN1465-6906
Aparece en las colecciones: (IBE) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
human_placental_transcriptome_Hughes.pdf3,2 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.