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Title

Carnitine palmitoyltransferase 1 increases lipolysis, UCP1 protein expression and mitochondrial activity in brown adipocytes

AuthorsCalderon-Dominguez, María; García-Casarrubios, Ester; Obregón, María Jesús; Valverde, Ángela M.; Herrero, Laura
Issue Date2016
PublisherPublic Library of Science
CitationPLoS ONE 11(7): e0159399 (2016)
AbstractThe discovery of active brown adipose tissue (BAT) in adult humans and the fact that it is reduced in obese and diabetic patients have put a spotlight on this tissue as a key player in obesity-induced metabolic disorders. BAT regulates energy expenditure through thermogenesis; therefore, harnessing its thermogenic fat-burning power is an attractive therapeutic approach. We aimed to enhance BAT thermogenesis by increasing its fatty acid oxidation (FAO) rate. Thus, we expressed carnitine palmitoyltransferase 1AM (CPT1AM), a permanently active mutant form of CPT1A (the rate-limiting enzyme in FAO), in a rat brown adipocyte (rBA) cell line through adenoviral infection. We found that CPT1AM-expressing rBA have increased FAO, lipolysis, UCP1 protein levels and mitochondrial activity. Additionally, enhanced FAO reduced the palmitate-induced increase in triglyceride content and the expression of obese and inflammatory markers. Thus, CPT1AM-expressing rBA had enhanced fat-burning capacity and improved lipid-induced derangements. This indicates that CPT1AM-mediated increase in brown adipocytes FAO may be a new approach to the treatment of obesity-induced disorders.
DescriptionThis is an open access article distributed under the terms of the Creative Commons Attribution License.-- et al.
Publisher version (URL)https://doi.org/10.1371/journal.pone.0159399
URIhttp://hdl.handle.net/10261/151168
DOI10.1371/journal.pone.0159399
Identifiersdoi: 10.1371/journal.pone.0159399
e-issn: 1932-6203
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