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Título

In vivo testing of gold nanoparticles using the Caenorhabditis elegans model organism

AutorGonzález Moragas, Laura ; Berto, Pascal; Vilches, Clara; Quidant, Romain; Kolovou, Androniki; Santarella-Mellwig, Rachel; Schwab, Yannick; Stürzenbaum, Stephen; Roig Serra, Anna ; Laromaine, Anna
Palabras claveBiological interactions
Caenorhabditis elegans
Digestive system
Enterocytes
Endocytosis
Gold nanoparticles
Fecha de publicación15-abr-2017
EditorElsevier
CitaciónActa Biomaterialia 53: 598-609 (2017)
ResumenGold nanoparticles (AuNPs) are present in many man-made products and cosmetics and are also used by the food and medical industries. Tight regulations regarding the use of mammalian animals for product testing can hamper the study of the specific interactions between engineered nanoparticles and biological systems. Invertebrate models, such as the nematode Caenorhabditis elegans (C. elegans), can offer alternative approaches during the early phases of nanoparticle discovery. Here, we thoroughly evaluated the biodistribution of 11-nm and 150-nm citrate-capped AuNPs in the model organism C. elegans at multiple scales, moving from micrometric to nanometric resolution and from the organismal to cellular level. We confirmed that the nanoparticles were not able to cross the intestinal and dermal barriers. We investigated the effect of AuNPs on the survival and reproductive performance of C. elegans, and correlated these effects with the uptake of AuNPs in terms of their number, surface area, and metal mass. In general, exposure to 11-nm AuNPs resulted in a higher toxicity than the larger 150-nm AuNPs. NP aggregation inside C. elegans was determined using absorbance microspectroscopy, which allowed the plasmonic properties of AuNPs to be correlated with their confinement inside the intestinal lumen, where anatomical traits, acidic pH and the presence of biomolecules play an essential role on NP aggregation. Finally, quantitative PCR of selected molecular markers indicated that exposure to AuNPs did not significantly affect endocytosis and intestinal barrier integrity.
Versión del editorhttp://dx.doi.org/10.1016/j.actbio.2017.01.080
URIhttp://hdl.handle.net/10261/150988
DOI10.1016/j.actbio.2017.01.080
ISSN1742-7061
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