English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/150918
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Species identification in mixed tuna samples with next-generation sequencing targeting two short cytochrome b gene fragments

AutorKappel, Kristina; Haase, Ilka; Käppel, Christine; González Sotelo, Carmen ; Schröder, Ute
Palabras claveTuna mixtures
Tuna tins
Next-generation sequencing
Authentication
Cytochrome b
Fecha de publicación2017
EditorElsevier
CitaciónFood Chemistry 234: 212-219 (2017)
ResumenConventional Sanger sequencing of PCR products is the gold standard for species authentication of seafood products. However, this method is inappropriate for the analysis of products that might contain mixtures of species, such as tinned tuna. The purpose of this study was to test whether next-generation sequencing (NGS) can be a solution for the authentication of mixed products. Nine tuna samples containing mixtures of up to four species were prepared and subjected to an NGS approach targeting two short cytochrome b gene (cytb) fragments on the Illumina MiSeq platform. Sequence recovery was precise and admixtures of as low as 1% could be identified, depending on the species composition of the mixtures. Duplicate samples as well as two individual NGS runs produced very similar results. A first test of three commercial tinned tuna samples indicated the presence of different species in the same tin, although this is forbidden by EU law
Descripción8 páginas, 3 figuras, 1 tabla
Versión del editorhttps://doi.org/10.1016/j.foodchem.2017.04.178
URIhttp://hdl.handle.net/10261/150918
DOI10.1016/j.foodchem.2017.04.178
ISSN0308-8146
Aparece en las colecciones: (IIM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Species_identification_mixed_2017.pdf Embargado hasta 1 de noviembre de 2018652,34 kBAdobe PDFVista previa
Visualizar/Abrir     Petición de una copia
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.