English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/150634
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Identification of peptides released from flaxseed (Linum usitatissimum) protein by Alcalase® hydrolysis: Antioxidant activity

AutorGuimarães Drummond e Silva, Fernanda; Hernández-Ledesma, Blanca ; Amigo, Lourdes ; Netto, Flavia Maria; Miralles, Beatriz
Palabras claveTandem mass spectrometry
Alcalase® hydrolysis
Antioxidant capacity
Peptides
Flaxseed
Fecha de publicación2017
EditorElsevier
CitaciónLWT - Food Science and Tecnology 76A: 140-146 (2017)
ResumenIn this study, the hydrolysis of a flaxseed protein isolate with Alcalase was performed as a strategy to generate antioxidant peptides. A chromatographic separation of the hydrolysate was conducted by RP-HPLC. Both hydrolysate and six collected fractions were subjected to ORAC and FRAP assays to evaluate their antioxidant capacity. The higher antioxidant values were shown by fractions containing predominantly low molecular weight peptides, as it was demonstrated by MALDI analysis. Four peptides were identified by LC-MS/MS and one by Edman degradation. The peptide with sequence GFPGRLDHWCASE was synthesised showing a notable ORAC activity, 3.20 μmol Trolox equivalents/μmol of peptide. This value was higher than that reported for butylated hydroxyanisole. Therefore, the contribution of this peptide to the activity of the fraction where it had been found was 61%. The identified sequences represent an advance in the molecular characterization of the flaxseed protein fraction.
Versión del editorhttps://doi.org/10.1016/j.lwt.2016.10.049
URIhttp://hdl.handle.net/10261/150634
DOI10.1016/j.lwt.2016.10.049
Identificadoresdoi: 10.1016/j.lwt.2016.10.049
issn: 0023-6438
Aparece en las colecciones: (CIAL) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Alcalasehydrolysis.pdf142,45 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.