Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/150523
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Visualizing dynamic microvillar search and stabilization during ligand detection by T cells |
Autor: | Cai, En; Marchuk, Kyle; Beemiller, Peter; Beppler, Casey; Rubashkin, Matthew G.; Weaver, Valerie M.; Gérard, Audrey; Liu, Tsung-Li; Chen, Bi-Chang; Betzig, Eric; Bartumeus, Frederic CSIC ORCID ; Krummel, Matthew F. | Fecha de publicación: | 2017 | Editor: | American Association for the Advancement of Science | Citación: | Science 356 : eaal3118 (2017) | Resumen: | During immune surveillance, T cells survey the surface of antigen-presenting cells. In searching for peptide-loaded major histocompatibility complexes (pMHCs), they must solve a classic trade-off between speed and sensitivity. It has long been supposed that microvilli on T cells act as sensory organs to enable search, but their strategy has been unknown.We used lattice light-sheet and quantum dot–enabled synaptic contact mapping microscopy to show that anomalous diffusion and fractal organization of microvilli survey the majority of opposing surfaces within 1 minute. Individual dwell times were long enough to discriminate pMHC half-lives and T cell receptor (TCR) accumulation selectively stabilized microvilli. Stabilization was independent of tyrosine kinase signaling and the actin cytoskeleton, suggesting selection for avid TCR microclusters. This work defines the efficient cellular search process against which ligand detection takes place. | Descripción: | Este artículo contiene10 páginas, 5 movies, 6 figuras. | Versión del editor: | http://dx.doi.org/10.1126/science.aal3118 | URI: | http://hdl.handle.net/10261/150523 | DOI: | 10.1126/science.aal3118 | ISSN: | 0036-8075 |
Aparece en las colecciones: | (CEAB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Cai_etal_Science17_PREPRINT.pdf | 55,44 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
118
checked on 23-feb-2024
SCOPUSTM
Citations
170
checked on 12-mar-2024
WEB OF SCIENCETM
Citations
162
checked on 24-feb-2024
Page view(s)
297
checked on 18-mar-2024
Download(s)
347
checked on 18-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.