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Title

Reduced β-lactoglobulin IgE binding upon in vitro digestion as a result of the interaction of the protein with casein glycomacropeptide

AuthorsMartínez, María J.; Martos, Gustavo ; Molina, Elena ; Pilosof, Ana M. R.
KeywordsInteractions
Allergenicity
Digestion
Issue Date2016
PublisherElsevier
CitationFood Chemistry 192: 943-949 (2016)
AbstractThe aim of this work was to evaluate the effect of the presence of casein glycomacropeptide (CMP) on the in vitro digestibility and potential allergenicity of β-lactoglobulin (β-lg)-CMP mixtures. The digestion products were analyzed by RP-HPLC and RP-HPLC-ESI-MS/MS. The potential allergenicity of the digestion products was studied by human IgE binding by inhibition ELISA with serum samples from children with clinical allergic symptoms to β-lg. No differences were observed by HPLC in the mixtures hydrolysates due to CMP-β-lg interactions. RP-HPLC-ESI-MS/MS results showed different peptides occurring in the mixtures hydrolysates. Additionally, it was observed a significant reduction of β-lg IgE binding in the presence of CMP. The disappearance of epitopes in the digested mixtures could explain the lower IgE binding observed in these systems compared to β-lg. It can be concluded that the presence of CMP in products containing β-lg may modify the digestion products that may reduce the potential allergenicity of β-lg.
URIhttp://hdl.handle.net/10261/150460
DOI10.1016/j.foodchem.2015.07.097
Identifiersdoi: 10.1016/j.foodchem.2015.07.097
issn: 0308-8146
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