English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/150124
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

A Comparative Study on Surface Treatments in the Immobilization Improvement of Hexahistidine-tagged Protein on the Indium Tin Oxide Surface

AuthorsIsmail, M.B.; Casañ Pastor, Nieves ; Pérez Soler, Estela ; Soltani, A; Othmane, A
KeywordsIndium tin oxide
Piranha treatment
Biofunctionalization
AFM
XPS
Histidine-tagged proteins
Electrochemistry
Indium tin oxide
Issue DateJan-2016
PublisherOMICS Publishing Group
CitationJournal of Nanomedicine & Nanotechnology 07(02): 1000372 (2016)
AbstractBiosensors are becoming increasingly important in various sectors. However, choosing transducer type and surface treatment still faces severe limitations. Indium-tin oxide is widely used in electrochemical sensing, though surface cleaning and functionalization are not always straightforward and often poorly controlled. Here, we have covalently immobilized a hexahistidine-tagged model protein on three different treated ITO surfaces using a Ni2+ chelator moiety. The study shows that compared to two other treatments (H2O/H2O2/NH4OH and KOH treatments), the ITO Piranha treatment gives satisfactory results in term of yields of EC12 protein immobilized on the surface. The study in detail of treatments effect was realized by applying a combination of local and global techniques such X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), Attenuated total reflectance Fourier transform infrared spectroscopy (FTIR-ATR), contact angle measurements, and electrochemical impedance spectroscopy (EIS). The biofunctionalization allowed proteins to move freely around their anchoring bond. As the polyhistidine tags are widely used in protein engineering, such substrates offer a large panel of applications. This covalent and oriented immobilization process of recombinant proteins applied to ITO could provide transparent electroactive surfaces of high quality for electrochemical detection of soluble compounds, as well as cells.
Publisher version (URL)http://dx.doi.org/10.4172/2157-7439.1000372
URIhttp://hdl.handle.net/10261/150124
DOI10.4172/2157-7439.1000372
ISSN2157-7439
Appears in Collections:(ICMAB) Artículos
Files in This Item:
File Description SizeFormat 
Ismail_JNanNan_2016_editorial.pdf873,76 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.