Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/150116
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | A feasibility study of UHPLC-HRMS accurate-mass screening methods for multiclass testing of organic contaminants in food |
Autor: | Pérez-Ortega, Patricia; Gilbert-López, Bienvenida CSIC ORCID; Molina-Díaz, Antonio | Palabras clave: | Food Screening methods LC-MS Pesticides |
Fecha de publicación: | 2016 | Editor: | Elsevier | Citación: | Talanta 160: 704-712 (2016) | Resumen: | The feasibility of accurate-mass multi-residue screening methods using liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS) using time-of-flight mass spectrometry has been evaluated, including over 625 multiclass food contaminants as case study. Aspects such as the selectivity and confirmation capability provided by HRMS with different acquisition modes (full-scan or full-scan combined with collision induced dissociation (CID) with no precursor ion isolation), and chromatographic separation along with main limitations such as sensitivity or automated data processing have been examined. Compound identification was accomplished with retention time matching and accurate mass measurements of the targeted ions for each analyte (mainly (de)protonated molecules). Compounds with the same nominal mass (isobaric species) were very frequent due to the large number of compounds included. Although 76% of database compounds were involved in isobaric groups, they were resolved in most cases (99% of these isobaric species were distinguished by retention time, resolving power, isotopic profile or fragment ions). Only three pairs could not be resolved with these tools. In-source CID fragmentation was evaluated in depth, although the results obtained in terms of information provided were not as thorough as those obtained using fragmentation experiments without precursor ion isolation (all ion mode). The latter acquisition mode was found to be the best suited for this type of large-scale screening method instead of classic product ion scan, as provided excellent fragmentation information for confirmatory purposes for an unlimited number of compounds. Leaving aside the sample treatment limitations, the main weaknesses noticed are basically the relatively low sensitivity for compounds which does not map well against electrospray ionization and also quantitation issues such as those produced by signal suppression due to either matrix effects from coeluting matrix or from coeluting analytes present in the standards solutions which often occur as they contain hundreds of the analytes included in the screening methods. | URI: | http://hdl.handle.net/10261/150116 | DOI: | 10.1016/j.talanta.2016.08.002 | Identificadores: | doi: 10.1016/j.talanta.2016.08.002 issn: 0039-9140 e-issn: 1873-3573 |
Aparece en las colecciones: | (CIAL) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
37
checked on 12-abr-2024
WEB OF SCIENCETM
Citations
32
checked on 28-feb-2024
Page view(s)
238
checked on 18-abr-2024
Download(s)
80
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.