Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/150013
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invite to open peer review
Title

H2A monoubiquitination in Arabidopsis thaliana is generally independent of LHP1 and PRC2 activity

AuthorsZhou, Yue; Romero-Campero, Francisco J. CSIC ORCID ; Gómez-Zambrano, Ángeles; Turck, Franzisca; Calonje, Myriam CSIC ORCID
KeywordsPolycomb group repression mechanism
PRC1
PRC2
H2AK121ub
H3K27me3
Arabidopsis thaliana
Issue Date2017
PublisherBioMed Central
CitationGenome Biology, 18:69 (2017)
AbstractBackground: Polycomb group complexes PRC1 and PRC2 repress gene expression at the chromatin level in eukaryotes. The classic recruitment model of Polycomb group complexes in which PRC2-mediated H3K27 trimethylation recruits PRC1 for H2A monoubiquitination was recently challenged by data showing that PRC1 activity can also recruit PRC2. However, the prevalence of these two mechanisms is unknown, especially in plants as H2AK121ub marks were examined at only a handful of Polycomb group targets. Results: By using genome-wide analyses, we show that H2AK121ub marks are surprisingly widespread in Arabidopsis thaliana, often co-localizing with H3K27me3 but also occupying a set of transcriptionally active genes devoid of H3K27me3. Furthermore, by profiling H2AK121ub and H3K27me3 marks in atbmi1a/b/c, clf/swn, and lhp1 mutants we found that PRC2 activity is not required for H2AK121ub marking at most genes. In contrast, loss of AtBMI1 function impacts the incorporation of H3K27me3 marks at most Polycomb group targets. Conclusions: Our findings show the relationship between H2AK121ub and H3K27me3 marks across the A. thaliana genome and unveil that ubiquitination by PRC1 is largely independent of PRC2 activity in plants, while the inverse is true for H3K27 trimethylation.
Publisher version (URL)http://dx.doi.org/10.1186/s13059-017-1197-z
URIhttp://hdl.handle.net/10261/150013
DOI10.1186/s13059-017-1197-z
Appears in Collections:(IBVF) Artículos




Files in This Item:
File Description SizeFormat
BioMed.pdf2,06 MBAdobe PDFThumbnail
View/Open
Show full item record

CORE Recommender

PubMed Central
Citations

41
checked on Apr 15, 2024

SCOPUSTM   
Citations

62
checked on Apr 22, 2024

WEB OF SCIENCETM
Citations

56
checked on Feb 29, 2024

Page view(s)

332
checked on Apr 22, 2024

Download(s)

241
checked on Apr 22, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Related articles:


This item is licensed under a Creative Commons License Creative Commons