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Synthesis and modification of polyurethane for immobilization of Thermomyces lanuginosus (TLL) lipase for ethanolysis of fish oil in solvent free system

AuthorsPereira Cipolatti, Eliane; Moreno-Pérez, Sonia; Souza, Lívia T. A.; Guisán, José Manuel ; Oliveira, Débora de; Pessela, Benevides C.
Ethyl esters
Issue Date2015
CitationJournal of Molecular Catalysis B: Enzymatic 122: 163-169 (2015)
AbstractWe report the synthesis of polyurethane (PU) with polyethylene glycol (PEG) by miniemulsion polymerization as technique to produce a support for enzyme immobilization and stabilization. In order to study the influence of molar weight and concentration of PEG in the enzyme-support interaction, different PEG (400, 4000 and 6000 Da) was used during the synthesis of PU-PEG support to immobilize Thermomyces lanuginosus lipase (TLL). The enzymatic derivatives were effectively used in the production of polyunsaturated fatty acids (PUFAs), particularly derivative TLL-PU-PEG6000. This derivative showed the best results in terms of Km (0.183 mM) and Vmax (45.79 mmol/min/mL), calculated by Lineweaver-Burk plot (double reciprocal plot) and catalytic efficient (250.22), 100% of yield immobilization and recovered activity, reaching the highest values to the ethyl esters production (260 mM ethyl esters. U-1) making this derivative a potential low cost catalyst for ethanolysis reactions in solvent free system
Identifiersdoi: 10.1016/j.molcatb.2015.09.006
e-issn: 1873-3158
issn: 1381-1177
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