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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Fernández-Alvarez, Ana Julia | es_ES |
dc.contributor.author | Alvarez, María Soledad | es_ES |
dc.contributor.author | Cucarella, Carme | es_ES |
dc.contributor.author | Casado, Marta | es_ES |
dc.date.accessioned | 2017-05-18T10:50:27Z | - |
dc.date.available | 2017-05-18T10:50:27Z | - |
dc.date.issued | 2010-02-09 | - |
dc.identifier.citation | Journal of Biological Chemistry 285(16):11765-74 (2010) | es_ES |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/10261/149935 | - |
dc.description | 10 páginas, 9 figuras | es_ES |
dc.description.abstract | Insulin-induced gene 2 (INSIG2) and its homolog INSIG1 encode closely related endoplasmic reticulum proteins that regulate the proteolytic activation of sterol regulatory element-binding proteins, transcription factors that activate the synthesis of cholesterol and fatty acids in animal cells. Several studies have been carried out to identify INSIG2 genetic variants associated with metabolic diseases. However, few data have been published regarding the regulation of INSIG2 gene expression. Two Insig2 transcripts have been described in rodents through the use of different promoters that produce different noncoding first exons that splice into a common second exon. Herein we report the cloning and characterization of the human INSIG2 promoter and the detection of an INSIG2-specific transcript homologous to the Insig2b mouse variant in human liver. Deletion analyses on 3 kb of 5'-flanking DNA of the human INSIG2 gene revealed the functional importance of a 350-bp region upstream of the transcription start site. Mutated analyses, chromatin immunoprecipitation assays, and RNA interference analyses unveiled the significance of an Ets-consensus motif in the proximal region and the interaction of the Ets family member SAP1a (serum response factor (SRF) accessory protein-1a) with this region of the human INSIG2 promoter. Moreover, our findings suggest that insulin activated the human INSIG2 promoter in a process mediated by phosphorylated SAP1a. Overall, these results map the functional elements in the human INSIG2 promoter sequence and suggest an unexpected regulation of INSIG2 gene expression in human liver. | es_ES |
dc.description.sponsorship | This work was supported by Ministry of Science and Innovation Grant SAF2006-06760 and the Carlos III Health Institute (Red Temática de Investigación Cooperativa en Enfermedades Cardiovasculares (RECAVA) RD06/0014/0025). Supported by Spanish Ministry of Education and Science Fellowship BES-2004-6151. Supported by Consejo Superior de Investigaciones Científicas Fellowship JAE-predoc. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Society for Biochemistry and Molecular Biology | es_ES |
dc.relation | MICIN/SAF2006-06760 | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Carbohydrate/Metabolism | es_ES |
dc.subject | Gene/Promoters | es_ES |
dc.subject | Transcription/Promoter | es_ES |
dc.subject | Transcription/TCF | es_ES |
dc.subject | Gene expression | es_ES |
dc.subject | INSIG | es_ES |
dc.subject | SREBP | es_ES |
dc.title | Characterization of the human insulin-induced gene 2 (INSIG2) promoter: the role of Ets-binding motifs. | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1074/jbc.M109.067447 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1074/jbc.M109.067447 | es_ES |
dc.identifier.e-issn | 1083-351X | - |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Red Temática de Investigación Cooperativa en Enfermedades Cardiovasculares (España) | es_ES |
dc.contributor.funder | Ministerio de Educación y Ciencia (España) | es_ES |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004837 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004587 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.pmid | 20145255 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.languageiso639-1 | en | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
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2010 J Biol Chem 285-11765.pdf | 2,11 MB | Adobe PDF | Visualizar/Abrir |
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