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http://hdl.handle.net/10261/149845
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Boer, Roeland | es_ES |
dc.contributor.author | Ruiz-Masó, José A. | es_ES |
dc.contributor.author | Rueda, Manuel | es_ES |
dc.contributor.author | Petoukhov, Maxim V. | es_ES |
dc.contributor.author | Machón, Cristina | es_ES |
dc.contributor.author | Svergun, Dmitri I. | es_ES |
dc.contributor.author | Orozco, Modesto | es_ES |
dc.contributor.author | Solar, Gloria del | es_ES |
dc.contributor.author | Coll, Miquel | es_ES |
dc.date.accessioned | 2017-05-17T09:26:16Z | - |
dc.date.available | 2017-05-17T09:26:16Z | - |
dc.date.issued | 2016-02-15 | - |
dc.identifier.citation | Scientific Reports 6:20915 (2016) | es_ES |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/10261/149845 | - |
dc.description | 13 p.-7 fig.-1 tab. | es_ES |
dc.description.abstract | DNA replication initiation is a vital and tightly regulated step in all replicons and requires an initiator factor that specifically recognizes the DNA replication origin and starts replication. RepB from the promiscuous streptococcal plasmid pMV158 is a hexameric ring protein evolutionary related to viral initiators. Here we explore the conformational plasticity of the RepB hexamer by i) SAXS, ii)sedimentation experiments, iii) molecular simulations and iv) X-ray crystallography. Combining these techniques, we derive an estimate of the conformational ensemble in solution showing that the C-terminal oligomerisation domains of the protein form a rigid cylindrical scaffold to which the N-terminal DNA-binding/catalytic domains are attached as highly flexible appendages, featuring multiple orientations. In addition, we show that the hinge region connecting both domains plays a pivotal role in the observed plasticity. Sequence comparisons and a literature survey show that this hinge region could exists in other initiators, suggesting that it is a common, crucial structural element for DNA binding and manipulation. | es_ES |
dc.description.sponsorship | This study was supported by the Ministerio de Economía y Competitividad (Grants BFU2008-02372/BMC; BFU2011-22588, BFU2014-53550 and Unidad de Excelencia Maria de Maeztu MDM-2014-0435 to MC; BIO2009-10964 and E-SCIENCE to MO;BFU2010-19597, PNEUMOTALK, and CSD2008-00013, INTERMODS, to GdS; Ramón and Cajal subprogramme RYC-2011-09071 to CM), the Generalitat de Catalunya (Grants 2014-SGR1309 to MC and SGR2009-1348 to MO),Fundación Marcelino Botín (MO) and the European Commission (Cooperation Project SILVER, GA No. 260644 to MC and SCALALIFE Project to MO). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature Publishing Group | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014-53550-P | - |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MDM-2014-0435 | - |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/260644 | - |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.title | Conformational plasticity of RepB, the replication initiator protein of promiscuous streptococcal plasmid pMV158 | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1038/srep20915 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1038/srep20915 | es_ES |
dc.identifier.e-issn | 2045-2322 | - |
dc.rights.license | https://creativecommons.org/licenses/by/4.0/ | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | es_ES |
dc.contributor.funder | Generalitat de Catalunya | es_ES |
dc.contributor.funder | Fundación Botín | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100002809 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100006373 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.pmid | 26875695 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.languageiso639-1 | en | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
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srep2016.pdf | Artículo principal | 1,97 MB | Adobe PDF | Visualizar/Abrir |
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