Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/149813
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invite to open peer review
Title

Development of human colonic microbiota in the computer-controlled dynamic SIMulator of the GastroIntestinal tract SIMGI

AuthorsBarroso, Elvira CSIC; Cueva, Carolina CSIC ORCID ; Peláez, Carmen CSIC ORCID ; Martínez-Cuesta, M. Carmen CSIC ORCID ; Requena, Teresa CSIC ORCID
KeywordsSIMGI
Butyrate
SCFA
Colon microbiota
Multicompartmental GIT model
Issue Date2015
PublisherElsevier
CitationLWT - Food Science and Tecnology 61(2): 283-289 (2015)
AbstractThis study presents a computer-controlled dynamic SIMulator of the GastroIntestinal tract, SIMGI, which was designed to simulate the complete processes of digestion and fermentation. The system includes three-stage culture reactors intended to simulate in vitro the microbial conditions of different regions of the human large intestine. The evolution and composition of the microbial community in the ascending (AC), transverse (TC) and descending colon (DC) vessels was evaluated by PCR-DGGE and quantitative PCR. An overall decrease in counts of Bifidobacterium and Prevotella and an increase of Enterobacteriaceae was observed between the inoculation with human faeces and the bacterial community stabilized in the colon vessels. Regarding microbial differentiation, Bacteroides counts were more representative of the AC and TC vessels than the DC compartment. Within the butyrate producer groups, a low occurrence of Clostridium leptum and Ruminoccocus was observed in the AC compartment. The net SCFA production was highest in the AC compartment, whereas the ammonium levels indicated that proteolysis occurred similarly throughout the entire colon compartments. The results observed in the in vitro model SIMGI indicate that it can be used as a tool for studying the effects of diet or food components on modulating the gut microbiota and its metabolic activity.
URIhttp://hdl.handle.net/10261/149813
DOI10.1016/j.lwt.2014.12.014
Identifiersdoi: 10.1016/j.lwt.2014.12.014
issn: 0023-6438
Appears in Collections:(CIAL) Artículos




Files in This Item:
File Description SizeFormat
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record

CORE Recommender

SCOPUSTM   
Citations

83
checked on May 23, 2024

WEB OF SCIENCETM
Citations

68
checked on Feb 25, 2024

Page view(s)

422
checked on May 25, 2024

Download(s)

116
checked on May 25, 2024

Google ScholarTM

Check

Altmetric

Altmetric


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.