English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/149690
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

A bioguided identification of the active compounds that contribute to the antiproliferative/cytotoxic effects of rosemary extract on colon cancer cells

AutorBorrás-Linares, Isabel; Lozano-Sánchez, Jesús; Cifuentes, Alejandro ; Micol, Vicente; Segura-Carretero, Antonio
Palabras claveTerpenoids
Colon cancer
HPLC-ESI-QTOF-MS
Antiproliferative activity
Cytotoxicity
Rosemary
Fecha de publicación2015
EditorElsevier
CitaciónFood and Chemical Toxicology 80: 215-222 (2015)
ResumenRosemary extracts have exhibited potential cytostatic or cytotoxic effects in several cancer cell models but their bioactive compounds are yet to be discovered. In this work, the anticancer activity of a rosemary-leaf extract and its fractions were assayed to identify the phenolic compounds responsible for their antiproliferative/cytotoxic effects on a panel of human colon cancer cell lines. Bioguided fractionation of the rosemary-leaf extract was achieved by semi-preparative chromatography. The rosemary extract and the compounds in the fractions were characterized and quantified by HPLC-ESI-QTOF-MS. Cellular viability in the presence of these fractions and the whole extract was determined after 24 or 48¿h incubations by using an MTT assay. Fractions containing diterpenes or triterpenes were the most active but not as much as the whole extract. In conclusion, carnosic acid, carnosol, 12-methoxycarnosic acid, taxodione, hinokione and betulinic acid were the putative candidates that contributed to the observed antiproliferative activity of rosemary in human colon cancer cells. Whether the effects of the extract and fractions are only cytostatic or cytotoxic needs to be elucidated. Nevertheless, the comparative antiproliferative study on the fractions and whole extract revealed potential synergistic effects between several components in the extract that may deserve further attention.
Descripciónet al.
Versión del editorhttps://doi.org/10.1016/j.fct.2015.03.013
URIhttp://hdl.handle.net/10261/149690
DOI10.1016/j.fct.2015.03.013
Identificadoresdoi: 10.1016/j.fct.2015.03.013
e-issn: 1873-6351
issn: 0278-6915
Aparece en las colecciones: (CIAL) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
bioguidedpost.pdf371,47 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.