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Evaluation of different immunological methods for the diagnosis of paratuberculosis in dairy cows and their relationship with the fecal shedding determined by PCR

AuthorsFernández Fernández, M. ; Mohamed, Y.; Fuertes, M. ; Castaño, Pablo ; Royo, M. ; Ferreras, Mª del Carmen ; Benavides, Julio ; Pérez Pérez, Valentín
KeywordsImmune-based test
Fecal excretion
Issue Date20-Jun-2016
PublisherInternational Association for Paratuberculosis
Citation13th International Colloquium on Paratuberculosis: P-03.20 (2016)
AbstractIntra-herd prevalence of Mycobacterium avium subsp. paratuberculosis (Map) infection has been evaluated in three different cattle dairy herds, of 107, 64 and 34 adult animals respectively, using immunological diagnostic methods. Their efficiency in the diagnosis of the infection and the relationship of their results with the age of the animals were also assessed. Animals from the herds were examined at a single time point by serum indirect ELISA, using Map PPA-3 protoplasmic as coating antigen of the plates, and by of IFN-a release assay in peripheral blood after incubation with avian PPD. Besides, the presence of specific antibodies in individual samples of milk and Map fecal excretion, evaluated by a real-time PCR (Paratb-kuanti VK), was assessed in all 34 animals from herd C. This study reflects a seroprevalence of 33.6%, 34.37% and 14.5% obtained in the three herds respectively, while 59%, 63.16% and 51.4% animals were positive to the IFN-a release test. It was clear the significant relationship existing between the age of the animals and the response to each diagnostic test employed. In this sense, the percentage of ELISA positive animals was significantly higher in adult cattle than in younger animals. In contrast, the number of cattle positive to IFN-a test was lower among adult cattle. In herd C, only 3 out of 34 cows were positive to ELISA in both milk and serum samples. Map fecal excretion was only found in the sole animal showing positive results to ELISA and IFN-a test plus clinical signs of paratuberculosis. According to these results, the number of Map infected animals determined by immunological methods in a herd with paratuberculosis can reach high values. Response to immune-based diagnostic tests is related to the age of the animals and would precede fecal Map excretion.
DescriptionTrabajo presentado al 13th International Colloquium on Paratuberculosis. Nantes (Francia), 20-24 junio, 2016.
Publisher version (URL)https://colloque.inra.fr/icp2016/Program
Appears in Collections:(IGM) Comunicaciones congresos
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