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Anthelmintic efficacy and cytotoxicity of nine plant species against the gastrointestinal nematode Teladorsagia circumcincta

AuthorsEsteban Ballesteros, M. ; González Lanza, Camino ; Gutiérrez-Corbo, C.; Balaña-Fouce, R.; Rojo Vázquez, Francisco Antonio ; Martínez Valladares, María
Issue Date13-Jul-2016
PublisherInternational Conference on Global Challenges in Neglected Tropical Diseases
Citation1st International Conference on Global Challenges in Neglected Tropical Diseases: PC35 (2016)
AbstractIn the present study the anthelmintic and cytotoxic activities of 9 plants was evaluated against Teladorsagia circumcincta, a helminth of sheep used as a model of gastrointestinal nematode. The anthelmintic activity was tested in vitro by the egg hatch assay (EHA). The EHA was performed with 200 µl of water containing 100-150 T. circumcincta eggs and 200 µl of the aqueous/methanolic plant extract, each at a final concentration ranged between 0.75 and 50 mg/ml. After 48 hours at 24°C the hatching percentage was calculated. The results of the effective concentrations required to inhibit 50% of egg hatching (ED50) (mg/ml) were the following ones for the aqueous extracts: 1.3 for Aesculus hippocastanum; 2.9 for Elettaria cardamomum; 1.8 for Chelidonium majus; 6.4 for Jasminum polyanthum; 2.4 for Sisymbrium irio and 1.5 for Isatis Tinctoria; and the following ones for the methanolic extracts: 7.3 for C. majus; 19.9 for I. tinctoria and 14.6 for J. polyanthum. The cytotoxicity of the different plant extracts was carried on murine J774 cell line. Briefly, 50.000 cells were treated with different concentrations of each plant extract for 72 hours in 96 well plates at 37ºC. Then, the viability of the cells was assessed using the Alamar Blue staining method. The median lethal dose (LC50) (mg/ml) values was calculated. Aqueous extracts of S. irio and J. polyanthum had the highest LC50, >5 and >1 mg/ml respectively, both extracts of C. majus the lowest values, 0.04 mg/ml. According to the selectivity index (SI), the aqueous extract of S. irio was the safest plant (≈1) and the extract with the worst SI was the methanolic extract of C. majus (0.00619).
DescriptionTrabajo presentado al 1st International Conference on Global Challenges in Neglected Tropical Diseases. León (Spain), 13-15 julio, 2016.
Appears in Collections:(IGM) Comunicaciones congresos
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