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dc.contributor.authorRomero-López, Cristina-
dc.contributor.authorBerzal-Herranz, Alfredo-
dc.identifierdoi: 10.3390/ph10010032-
dc.identifier.citationPharmaceuticals 10 (1): 32 (2017)-
dc.description.abstract<span style="letter-spacing: -0;">Aptamers are short DNA or RNA oligonucleotides specialized in the specific and efficient binding</span> <span style="letter-spacing: -0;">to a target molecule. They are obtained by in vitro selection or evolution processes. It was in 1990 that two independent research groups described the bases of a new in vitro<em> </em>technology for the identification</span> <span style="letter-spacing: -0;">of RNA molecules able to specifically bind to a target </span><span style="letter-spacing: -0;">[1,2]</span><span style="letter-spacing: -0;">. Tuerk and Gold established the principals of the in vitro selection process that was named SELEX (Systematic Evolution of Ligands by Exponential</span> enrichment), which is based on iterative cycles of binding, partitioning, and amplification of oligonucleotides from a pool of variant sequences [2]. Ellington and Szostak coined the term aptamer<em> </em><span style="letter-spacing: -0;">to define the selected molecules by the application of this method </span><span style="letter-spacing: -0;">[1]</span><span style="letter-spacing: -0;">. To date, numerous reports have described the isolation of aptamers directed against a great variety of targets covering a wide diversity</span> of molecules varying in nature, size, and complexity ranging from ions to whole cells, including small molecules (e.g., aminoacids, nucleotides, antibiotics), peptides, proteins, nucleic acids, and viruses, among others (for example, see [3–6]). Modifications and optimization of the SELEX procedure aimed to get newly modified aptamers has also attracted much interest (examples can be found in [7,8]). These advances along with the parallel progresses in the nucleic acids chemistry and cellular delivery fields have allowed for the rise of a new hope in developing aptamers as efficient molecular tools for diagnostics and therapeutics (for recent comprehensive reviews, see [9–11]).<br />-
dc.publisherMultidisciplinary Digital Publishing Institute-
dc.relation.isversionofPublisher's version-
dc.titleAptamers: Biomedical Interest and Applications-
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)-
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