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Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster

AuthorsBaenas, Nieves ; Piegholdt, Stefanie; Schloesser, Anke; Moreno, Diego A. ; García-Viguera, Cristina ; Rimbach, Gerald; Wagner, Anika E.
Issue Date18-Feb-2016
PublisherMultidisciplinary Digital Publishing Institute
CitationInternational Journal of Molecular Sciences 17 (2): 251 (2016)
AbstractWe used <i>Drosophila melanogaster</i> as a model system to study the absorption, metabolism and potential health benefits of plant bioactives derived from radish sprouts (<i>Raphanus sativus</i> cv. Rambo), a <i>Brassicaceae</i> species rich in glucosinolates and other phytochemicals. Flies were subjected to a diet supplemented with lyophilized radish sprouts (10.6 g/L) for 10 days, containing high amounts of glucoraphenin and glucoraphasatin, which can be hydrolyzed by myrosinase to the isothiocyanates sulforaphene and raphasatin, respectively. We demonstrate that <i>Drosophila melanogaster</i> takes up and metabolizes isothiocyanates from radish sprouts through the detection of the metabolite sulforaphane-cysteine in fly homogenates. Moreover, we report a decrease in the glucose content of flies, an upregulation of <i>spargel</i> expression, the <i>Drosophila</i> homolog of the mammalian PPARγ-coactivator 1 α, as well as the inhibition of α-amylase and α-glucosidase <i>in vitro</i>. Overall, we show that the consumption of radish sprouts affects energy metabolism in <i>Drosophila melanogaster</i> which is reflected by lower glucose levels and an increased expression of <i>spargel</i>, a central player in mitochondrial biogenesis. These processes are often affected in chronic diseases associated with aging, including type II diabetes mellitus.
Publisher version (URL)http://doi.org/10.3390/ijms17020251
Identifiersdoi: 10.3390/ijms17020251
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