English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/149188
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster

AutorBaenas, Nieves ; Piegholdt, Stefanie; Schloesser, Anke; Moreno, Diego A. ; García-Viguera, Cristina ; Rimbach, Gerald; Wagner, Anika E.
Fecha de publicación18-feb-2016
EditorMultidisciplinary Digital Publishing Institute
CitaciónInternational Journal of Molecular Sciences 17 (2): 251 (2016)
ResumenWe used <i>Drosophila melanogaster</i> as a model system to study the absorption, metabolism and potential health benefits of plant bioactives derived from radish sprouts (<i>Raphanus sativus</i> cv. Rambo), a <i>Brassicaceae</i> species rich in glucosinolates and other phytochemicals. Flies were subjected to a diet supplemented with lyophilized radish sprouts (10.6 g/L) for 10 days, containing high amounts of glucoraphenin and glucoraphasatin, which can be hydrolyzed by myrosinase to the isothiocyanates sulforaphene and raphasatin, respectively. We demonstrate that <i>Drosophila melanogaster</i> takes up and metabolizes isothiocyanates from radish sprouts through the detection of the metabolite sulforaphane-cysteine in fly homogenates. Moreover, we report a decrease in the glucose content of flies, an upregulation of <i>spargel</i> expression, the <i>Drosophila</i> homolog of the mammalian PPARγ-coactivator 1 α, as well as the inhibition of α-amylase and α-glucosidase <i>in vitro</i>. Overall, we show that the consumption of radish sprouts affects energy metabolism in <i>Drosophila melanogaster</i> which is reflected by lower glucose levels and an increased expression of <i>spargel</i>, a central player in mitochondrial biogenesis. These processes are often affected in chronic diseases associated with aging, including type II diabetes mellitus.
Versión del editorhttp://doi.org/10.3390/ijms17020251
URIhttp://hdl.handle.net/10261/149188
DOI10.3390/ijms17020251
Identificadoresdoi: 10.3390/ijms17020251
Aparece en las colecciones: (CEBAS) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
ijms-17-00251.pdf1,29 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.