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The Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA

AutorAviñó, Anna; Mazzini, Stefania; Gargallo, Raimundo; Eritja Casadellà, Ramón
Fecha de publicación5-feb-2016
EditorMultidisciplinary Digital Publishing Institute
CitaciónInternational Journal of Molecular Sciences 17 (2): 211 (2016)
ResumenTriplex stability is studied in crowding conditions using small cosolutes (ethanol, acetonitrile and dimethylsulfoxide) by ultraviolet (UV), circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopies. The results indicate that the triplex is formed preferentially when the triplex forming oligonucleotide (TFO) is RNA. In addition, DNA triplexes (D:D·D) are clearly less stable in cosolute solutions while the stability of the RNA triplexes (R:D·D) is only slightly decreased. The kinetic of triplex formation with RNA-TFO is slower than with DNA-TFO and the thermal stability of the triplex is increased with the salt concentration in EtOH-water solutions. Accordingly, RNA could be considered a potential molecule to form a stable triplex for regulatory purposes in molecular crowding conditions.
Versión del editorhttps://doi.org/10.3390/ijms17020211
URIhttp://hdl.handle.net/10261/149160
DOI10.3390/ijms17020211
Identificadoresdoi: 10.3390/ijms17020211
Aparece en las colecciones: (IQAC) Artículos
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