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The Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA

AuthorsAviñó, Anna ; Mazzini, Stefania; Gargallo, Raimundo; Eritja Casadellà, Ramón
Issue Date5-Feb-2016
PublisherMultidisciplinary Digital Publishing Institute
CitationInternational Journal of Molecular Sciences 17 (2): 211 (2016)
AbstractTriplex stability is studied in crowding conditions using small cosolutes (ethanol, acetonitrile and dimethylsulfoxide) by ultraviolet (UV), circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopies. The results indicate that the triplex is formed preferentially when the triplex forming oligonucleotide (TFO) is RNA. In addition, DNA triplexes (D:D·D) are clearly less stable in cosolute solutions while the stability of the RNA triplexes (R:D·D) is only slightly decreased. The kinetic of triplex formation with RNA-TFO is slower than with DNA-TFO and the thermal stability of the triplex is increased with the salt concentration in EtOH-water solutions. Accordingly, RNA could be considered a potential molecule to form a stable triplex for regulatory purposes in molecular crowding conditions.
Publisher version (URL)https://doi.org/10.3390/ijms17020211
Identifiersdoi: 10.3390/ijms17020211
Appears in Collections:(IQAC) Artículos
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