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Title

Spermadhesin PSP-I/PSP-II heterodimer induces migration of polymorphonuclear neutrophils into the uterine cavity of the sow

AuthorsRodriguez-Martinez, H.; Saravia, F.; Wallgren, M.; Martinez, E. A.; Sanz, Libia ; Roca, J.; Vazquez, J. M.; Calvete, Juan J.
KeywordsSeminal plasma
Spermadhesins
PMNs
Inflammation
T cells
Endometrium
In vivo
Pig
Issue Date30-Nov-2009
PublisherElsevier
CitationJournal of Reproductive Immunology 84(1):57-65 (2010)
AbstractSeminal plasma (SP) is a complex fluid which exerts biological actions in the female reproductive tract. In pigs, SP elicits endometrial inflammation and consequent immune changes after mating. This study tested whether heparin-binding spermadhesins (HBPs) and the heterodimer of porcine sperm adhesions I and II (PSP-I/PSP-II) in SP recruit different lymphocyte subsets (CD2(+), CD4(+) and CD8(+) T cells) or polymorphonuclear leukocytes (PMNs) to the superficial endometrium or luminal epithelium and lumen, respectively, of oestrous sows. In Experiment 1, endometrial biopsies were taken between 2 and 120 min after infusion of uterine horns with HBPs, PSP-I/PSP-II or saline and evaluated by immunohistochemistry or histology. In Experiment 2, the uterus of oestrous sows was infused with PSP-I/PSP-II or saline to assess PMN numbers in the uterine lumen 3h later. PSP-I/PSP-II elicited CD2+ T cell recruitment from 10 min, and CD8(+) T cells from 60 min after infusion, while HBPs increased CD4(+) T cell recruitment by 120 min. PSP-I/PSP-II but not HBPs induced PMN migration to the surface epithelium by 10 min. PMN numbers were elevated 5-fold by 30 min and 7-fold from 60 min, with PMNs detectable in the lumen from 30 min after infusion. Six-fold more PMNs were collected from the uterine lumen of PSP-I/PSP-II-infused sows compared to controls at 3h after infusion. These data show that PSP-I/PSP-II heterodimer in seminal plasma has a predominant role in triggering the recruitment of uterine PMNs and T cells after mating, initiating a cascade of transient and long-lasting immunological events.
Description9 páginas, 4 figuras.
Publisher version (URL)http://dx.doi.org/10.1016/j.jri.2009.10.007
URIhttp://hdl.handle.net/10261/149148
DOI10.1016/j.jri.2009.10.007
ISSN0165-0378
E-ISSN1872-7603
Appears in Collections:(IBV) Artículos
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