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http://hdl.handle.net/10261/149131
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dc.contributor.author | Hernández Sánchez, Karel | es_ES |
dc.contributor.author | Bujons, Jordi | es_ES |
dc.contributor.author | Joglar Tamargo, Jesús | es_ES |
dc.contributor.author | Charnock, Simon J. | es_ES |
dc.contributor.author | Domínguez de María, Pablo | es_ES |
dc.contributor.author | Fessner, Wolf Dieter | es_ES |
dc.contributor.author | Clapés Saborit, Pere | es_ES |
dc.date.accessioned | 2017-05-05T08:52:32Z | - |
dc.date.available | 2017-05-05T08:52:32Z | - |
dc.date.issued | 2017-02 | - |
dc.identifier.citation | ACS Catalysis 7(3): 1707–1711(2017) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/149131 | - |
dc.description.abstract | Amino acids are of paramount importance as chiral building blocks of life, for drug development in modern medicinal chemistry, and for the manufacture of industrial products. In this work, the stereoselective synthesis of (S)- and (R)-2-amino-4-hydroxybutanoic acid was accomplished using a systems biocatalysis approach comprising a biocatalytic one-pot cyclic cascade by coupling of an aldol reaction with an ensuing stereoselective transamination. A class II pyruvate aldolase from E. coli, expressed as a soluble fusion protein, in tandem with either an S- or R-selective, pyridoxal phosphate dependent transaminase was used as a catalyst to realize the conversion, with formaldehyde and alanine being the sole starting materials. Interestingly, the class II pyruvate aldolase was found to tolerate formaldehyde concentrations of up to 1.4 M. The cascade system was found to reach product concentrations for (S)- or (R)-2-amino-4-hydroxybutanoic acid of at least 0.4 M, rendering yields between 86% and >95%, respectively, productivities of >80 g L–1 d–1, and ee values of >99%. | es_ES |
dc.description.sponsorship | This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement no. 635595 (CarbaZymes), the Ministerio de Economía y Competitividad (MINECO), the Fondo Europeo de Desarrollo Regional (FEDER) (grant no. CTQ2015-63563-R to P.C.), and COST action CM1303 Systems Biocatalysis. | es_ES |
dc.description.sponsorship | We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI). | - |
dc.language.iso | eng | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/635595 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-63563-R | - |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Aldolases | es_ES |
dc.subject | Biocatalysis | es_ES |
dc.subject | Green chemistry | es_ES |
dc.subject | Substrate cycling | es_ES |
dc.subject | Transaminases | es_ES |
dc.title | Combining aldolases and transaminases for the synthesis of 2‑amino-4-hydroxybutanoic acid | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1021/acscatal.6b03181 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | https://doi.org/10.1021/acscatal.6b03181 | es_ES |
dc.identifier.e-issn | 2155-5435 | - |
dc.rights.license | http://pubs.acs.org/page/policy/authorchoice_termsofuse.html | - |
dc.contributor.funder | European Research Council | es_ES |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI) | - |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000781 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.languageiso639-1 | en | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
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ACS_catalysis_2017_1707.pdf | 923,27 kB | Adobe PDF | Visualizar/Abrir |
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