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dc.contributor.authorArranz-Solís, D.-
dc.contributor.authorBenavides, Julio-
dc.contributor.authorRegidor-Cerrillo, Javier-
dc.contributor.authorHorcajo, P.-
dc.contributor.authorCastaño, Pablo-
dc.contributor.authorFerreras, Mª del Carmen-
dc.contributor.authorJiménez Pelayo, L.-
dc.contributor.authorCollantes Fernández, E.-
dc.contributor.authorFerre, Ignacio-
dc.contributor.authorHemphill, A.-
dc.contributor.authorPérez Pérez, Valentín-
dc.contributor.authorOrtega Mora, Luis M.-
dc.identifier.citationVeterinary Research 47: 2 (2016)-
dc.description13 páginas, 4 Figuras.--Creative Commons Attribution License-
dc.description.abstractBesides its importance in cattle, Neospora caninum may also pose a high risk as abortifacient for small ruminants. We have recently demonstrated that the outcome of experimental infection of pregnant sheep with 106 Nc-Spain7 tachy‑ zoites is strongly dependent on the time of gestation. In the current study, we assessed peripheral and local immune response in those animals. Serological analysis revealed earlier and higher IFN-γ and IgG responses in ewes infected at early (G1) and mid (G2) gestation, when abortion occurred. IL-4 was not detected in sera from any sheep. Inflam‑ matory infiltrates in the placenta mainly consisted of CD8+ and, to a lesser extent, CD4+ T cells and macrophages (CD163+). The infiltrate was more intense in sheep infected at mid-gestation. In the foetal mesenchyme, mostly free tachyzoites were found in animals infected at G1, while those infected in G2 displayed predominantly particulate antigen, and parasitophorous vacuoles were detected in sheep infected at G3. A similar pattern of placental cytokine mRNA expression was found in all groups, displaying a strengthened upregulation of IFN-γ and IL-4 and milder increases of TNF-α and IL-10, reminiscent of a mixed Th1 and Th2 response. IL-12 and IL-6 were only slightly upregu‑ lated in G2, and TGF-β was downregulated in G1 and G2, suggestive of limited T regulatory (Treg) cell activity. No significant expression of TLR2 or TLR4 could be detected. In summary, this study confirms the pivotal role of systemic and local immune responses at different times of gestation during N. caninum infection in sheep-
dc.description.sponsorshipWe gratefully acknowledge Esperanza Selva from the SALUVET group (Complutense University of Madrid, Spain), Mario Ferrero, Melchor Molero, Iván Panero, Miguel Fernández and Carmen Espiniella from IGM (CSIC-ULE) and Idoia Badiola, from Agropal S. C. for their excellent technical assistance. D. Arranz is supported by a fellowship from the Spanish Ministry of Economy and Competitiveness (MINECO), as part of the Program of Training of Researcher Staff (FPI, grant number BES-2011-043433). This work was supported by the Swiss National Foundation (SNF grant No. 310030_146162), research grant LE253U13 from Junta de Castilla y León (Spain) and the Iberoamerican Science and Technology Programme for Development (CYTED, 113rt0469).-
dc.publisherBioMed Central-
dc.relation.isversionofPublisher's version-
dc.titleSystemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation-
dc.description.versionPeer Reviewed-
dc.rights.licenseCreative Commons Attribution License-
dc.contributor.funderJunta de Castilla y León-
dc.contributor.funderPrograma Iberoamericano de Ciencia y Tecnología para el Desarrollo-
dc.contributor.funderSwiss National Science Foundation-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
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