English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/148949
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


The opioid peptide beta-endorphin stimulates acrosome reaction in human spermatozoa

AuthorsUrizar, Itziar; Estomba, Haizea; Muñoa, Iraia; Matorras, Roberto; Esposito, Antonia; Candenas, M. Luz ; Pinto Pérez, Francisco M. ; Valdivia, Asier; Irazusta, Jon; Subiran, Nerea
KeywordsSignalling pathways
Acrosome reaction
Male fertility
Opioid peptides
Issue Date2016
CitationAndrology 4: 143- 151 (2016)
AbstractThe acrosome reaction occurs in vivo following sperm capacitation and is essential for the acquisition of sperm fertilization ability. However, little is known about the molecular identity of the physiological acrosome reaction regulators. In addition to progesterone, which is produced by cumulus oophorus cells and known to regulate acrosome reaction by activating the specific calcium channel CatSper, endogenous opioid peptides such as beta-endorphin and met-enkephalin are present at high concentrations in the follicular fluid suggesting that the opioid system may be involved in the mechanisms regulating the acrosome reaction in humans. By using Reverse Transcription-PCR, western blot and immunofluorescence approaches, we described the presence and localization of the beta-endorphin precursor, pro-opiomelanocortinin the middle section and in flagellum of human spermatozoa, and inside the seminiferous tubules of human testis. Flow cytometry and intracellular calcium analyses showed that beta-endorphin causes an inversely dose-dependent increase in the percentage of acrosome-reacted sperm cells by a calcium-independent protein kinase C pathway. These findings are important for future studies of sperm physiology and provide new insight into the function of the opioid system as a target of fertility management.
Identifiersdoi: 10.1111/andr.12133
issn: 2047-2927
Appears in Collections:(IIQ) Artículos
Files in This Item:
File Description SizeFormat 
2015-Urizar et al Andrology-ULTIMA VERSION-enviada a CSIC digital-Abril2017.pdf374,87 kBAdobe PDFThumbnail
Figuras-Urizar et al-ULTIMAS-Andrology.pptx1,04 MBMicrosoft Powerpoint XMLView/Open
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.