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Killing effect of R-pyocins on susceptible and multiresistant Pseudomonas aeruginosa clinical isolates

AuthorsRedero, Mar; López-Causapé, Carla; Basas, Jana; Gavaldà, Joan; Oliver, Antonio; Blázquez Gómez, Jesús ; Prieto Márquez, Ana Isabel
Issue DateSep-2016
CitationXI Reunión del Grupo de Microbiología Molecular de la Sociedad Española de Microbiología (2016)
AbstractThe recent appearance and dissemination of multidrug resistance (MDR) among bacteria have created a new threat for which traditional antibiotics offer little protection. After decades of neglect, the phage-therapy is beginning to be considered as a possible reemerging alternative. R-type pyocins are high-molecular-weight bacteriocins encoded in the chromosome of some bacterial species, such as Pseudomonas aeruginosa, supposedly to defend themselves against other strains of the same species. R-pyocins resemble the tails of lysogenic Myoviridae bacteriophages from which probably they evolved. The development of pulmonary MDR-P. aeruginosa infections is one of the most serious complications in the course of disease in patients with cystic fibrosis (CF), which often have very few antibiotic options . The analysis of approximately 200 P. aeruginosa clinical isolates shows that CF P. aeruginosa strains are hyper-susceptible to R-pyocins compared to isolates from other sources (bronchial aspirates, bloodstream, skin, sputum, abdominal, urine). The efficacy of R-pyocins in the eradication of P. aeruginosa infections has been tested through in vitro biofilms experiments and animal models. Treatments with R1, R2, or R5 pyocins prevent biofilm formation and also displayed a strong antimicrobial activity against established biofilms. In addiction, R-pyocins were also effective in ex vivo pig lung infection experiments, showing a 1000-fold decrease in the number of infecting bacteria. Moreover, administration of R2 pyocins in an in vivo murine model of ventilator-associated pneumonia (VAP) with a MDR clinical isolate (Pa1016) also showed a reduction of 100- fold in the number of bacteria recovered from lung after 24h of infection. Altogether, these results strongly suggest that R-pyocins could be a possible alternative to be used as antimicrobial agent alone or in combination with antibiotic therapy for the treatment of P. aeruginosa infections.
DescriptionPóster presentado en la XI Reunión del Grupo de Microbiología Molecular de la Sociedad Española de Microbiología (SEM), celebrada en Sevilla del 6 al 8 de septiembre de 2016.
Appears in Collections:(IBIS) Comunicaciones congresos
(CNB) Comunicaciones congresos
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