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Does aggregation change S. aureus physiology?

AuthorsDorado, Pedro; García, Begoña ; Valle Turrillas, Jaione ; Solano Goñi, Cristina ; Lasa, Íñigo
Issue DateSep-2016
CitationXI Reunión del Grupo de Microbiología Molecular de la Sociedad Española de Microbiología (2016)
AbstractBacterial aggregation can occur when bacteria come into contact with a surface during biofilm development and also while bacteria are freely swimming in liquid media. Bacterial aggregation usually correlates with the production of exopolysaccharides and/or specific surface proteins. In this work, we explore the concept that the contact between bacteria imposed by the aggregation process causes specific changes in bacterial physiology. The concept of sensing bacterial contact implies the existence of an apparatus that allows the perception of neighboring bacteria and provokes a biochemical cascade following the contact with other bacteria inside the aggregates. Staphylococcus aureus, a commensal and pathogenic microorganism responsible for a wide range of serious acute and chronic diseases in humans and animals, has the capacity to form aggregates when bacteria overexpresses either the exopolysaccharide PIA/PNAG or surface proteins such as SasG and Bap. Bap is a high molecular weight surface protein that connects staphylococcal cells through a self-assembly process resulting in the formation of amyloid-like aggregates. In this study we have used the Bap mediated bacterial aggregation process to identify the physiological changes undergone by S. aureus in the presence of Bap amyloid fibers. We have compared total protein extracts obtained from aggregated and planktonic bacteria using a differential proteomic analysis (label-free). Analysis of the proteomic results revealed that more than 200 proteins were differentially expressed when both conditions were compared, including global regulators and twocomponent signal transduction systems. Aggregation repressed the expression of proteins involved in peptidoglycan and cell wall synthesis, purine and pentose biosynthesis and recruitment and regulation of iron, whereas it induced the expression of proteins involved in DNA repair, genome stability, and teichoic acid biosynthesis amongst others. These results will be discussed in the presentation.
DescriptionTrabajo presentado en la XI Reunión del Grupo de Microbiología Molecular de la Sociedad Española de Microbiología (SEM), celebrada en Sevilla del 6 al 8 de septiembre de 2016.
Appears in Collections:(IDAB) Comunicaciones congresos
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