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Horizontal and vertical transmission of the hypovirulence-associated Mycovirus Fusarium oxysporum f. sp. dianthi virus 1

AutorLemus-Minor, C.G.; Cañizares, M. Carmen; García-Pedrajas, María D.; Pérez-Artés, Encarnación
Fecha de publicaciónsep-2016
CitaciónXVIII Congreso de la Sociedad Española de Fitopatología (2016)
ResumenFusarium oxysporum f. sp. dianthi (Fod) is the causal agent of carnation wilt, the most devastating disease of this plant species. Recently, a “chryso-like” mycovirus, named Fusarium oxysporum f. sp. dianthi virus 1 (FodV1), has been identified in isolate Fod 116 of Fod. FodV1 genome has been fully sequenced, and it consists of 4 dsRNA segments ranging from 2.6 to 3.5 kb. Comparison of the naturally infected Fod 116 isolate (Fod 116V+) and a selected version with a very reduced level of viral dsRNA (Fod 116V-), evidenced that FodV1 significantly reduced the mycelial growth and conidiation rates, and the virulence on carnation of his fungal host, thus opening the possibility to its use as a biological control agent. In this work we have demonstrated that FodV1 can be transferred horizontally, through hyphal anastomosis between vegetatively compatible isolates, and vertically during conidiation, and that the new recipient isolate reproduces the same phenotypic alterations that the donor one. To assess the horizontal transmission, isolate Fod 77, compatible with the donor Fod116V+, was transformed with the hph gen (hygromycin resistant), and after hyphal anastomosis between both isolates, the presence of FodV1 in the new fungal host was evidenced by dsRNA purification and RT-qPCR. Comparative analysis of isolate Fod 77 with (V+) and without (V-) mycovirus FodV1, showed significant alterations in all the hypovirulence-associated traits analyzed. The frequency of mycovirus transmission through asexual spores has also been determined, showing significant differences between the two virus-containing isolates.
DescripciónPóster presentado en el XVIII Congreso de la Sociedad Española de Fitopatología (SEF), celebrado en Palencia del 20 al 23 de septiembre de 2016.
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