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Characterization of an unclassified Mycovirus from Verticillium Dahliae and analysis of its transmision between defoliating and non-defoliating isolates

AutorCañizares, M. Carmen; Ostos, E.; López-Escudero, Francisco Javier; Pérez-Artés, Encarnación ; García-Pedrajas, María D.
Fecha de publicaciónsep-2016
CitaciónXVIII Congreso de la Sociedad Española de Fitopatología (2016)
ResumenThe soilborne pathogen Verticillium dahliae causes vascular wilts in a wide variety of crops. Isolates infecting olive can be classified into defoliating (D) or non-defoliating (ND). The spread of the D pathotype have greatly increased the impact of this disease on olive production. Fungal viruses (mycoviruses) from pathogenic species have potential as biocontrol agents as some reduce the virulence of their fungal host. The successful application of micoviruses to the control of fungi is dependent on their efficient transmission among isolates in natural fungal populations. Mycoviruses are thought to be transmitted horizontally mainly between somatically compatible isolates during hyphal anastomosis. In V. dahliae all D isolates belong to the same VCG while ND isolates conform different VCGs. In our aim to explore the potential of mycovirus to control Verticillium wilt, we have tested 200 isolates of V. dahliae from different areas of the Guadalquivir Valley and sampled in different years, revealing that only three exhibited viral infection. All three isolates harbored the same unclassified mycovirus with similarity to plant viruses of the family Tombusviridae which we designated VdUV1. Intriguingly, the VdUV1-infected isolates did not belong to the same VCG; two were ND and one D. An analysis of VdUV1 transmission by hyphal anastomosis using hygR-tagged D and ND recipient isolates showed that, as expected, it was readily transmitted among isolates of the same VCG while somatic incompatibility generally acted as a barrier for this transmission. However, we also found that the presence of “bridge” isolates can add complexity to this simple scenario. Thus, the two ND isolates transmitted VdUV1 only to other compatible ND isolates. By contrast the VdUV1-infected D isolate transmitted it to both D and ND isolates, although with clearly different efficiencies, 100% and 30%, respectively. Currently, we are investigating the putative effect of VdUV1 in virulence.
DescripciónPóster presentado en el XVIII Congreso de la Sociedad Española de Fitopatología (SEF), celebrado en Palencia del 20 al 23 de septiembre de 2016.
Aparece en las colecciones: (IAS) Comunicaciones congresos
(IHSM) Comunicaciones congresos
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