English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/148465
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy

AuthorsSpinato, Cinzia; Kierkowicz, Magdalena; Pach, Elzbieta; Martincic, Markus; Ballesteros, Belén ; Tobias, Gerard ; Bianco, Alberto
KeywordsSingle-walled carbon nanotubes
Filling
Samarium
Lutetium
Drug delivery
Radiotherapy
Antibody targeting
Cancer therapy
Issue Date7-Jul-2016
PublisherRoyal Society of Chemistry (Great Britain)
CitationNanoscale 8(25): 12626-12638 (2016)
AbstractIn the present work we have devised the synthesis of a novel promising carbon nanotube carrier for the targeted delivery of radioactivity, through a combination of endohedral and exohedral functionalization. Steam-purified single-walled carbon nanotubes (SWCNTs) have been initially filled with radioactive analogues (i.e. metal halides) and sealed by high temperature treatment, affording closed-ended CNTs with the filling material confined in the inner cavity. The external functionalization of these filled CNTs was then achieved by nitrene cycloaddition and followed by the derivatization with a monoclonal antibody (Cetuximab) targeting the epidermal growth factor receptor (EGFR), overexpressed by several cancer cells. The targeting efficiency of the so-obtained conjugate was evaluated by immunostaining with a secondary antibody and by incubation of the CNTs with EGFR positive cells (U87-EGFR+), followed by flow cytometry, confocal microscopy or elemental analyses. We demonstrated that our filled and functionalized CNTs can internalize more efficiently in EGFR positive cancer cells.
DescriptionSpinato, Cinzia et al.
Publisher version (URL)http://dx.doi.org/10.1039/C5NR07923C
URIhttp://hdl.handle.net/10261/148465
DOI10.1039/C5NR07923C
ISSN2040-3364
Appears in Collections:(ICMAB) Artículos
(CIN2) Artículos
Files in This Item:
File Description SizeFormat 
Spinato_Nanoscale_2016_postprint.pdf823,21 kBAdobe PDFThumbnail
View/Open
Show full item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.