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Title

Potato virus Y HCPro suppression of antiviral silencing in Nicotiana benthamiana plants correlates with its ability to bind in vivo to 21- and 22-nucleotide small RNAs of viral sequence

Other TitlesPVY HCPro binds in vivo 21-22 nt viral small RNAs
AuthorsDel Toro, Francisco ; Donaire, Livia ; Aguilar, Emmanuel; Chung, Bong-Nam; Tenllado, Francisco ; Canto, Tomás
Issue Date5-Apr-2017
PublisherAmerican Society for Microbiology
CitationJournal of Virology (2017)
AbstractWe have investigated short and small RNAs (sRNAs) that were bound to a biologically active hexahistidine-tagged Potato virus Y (PVY) HCPro suppressor of silencing, expressed from a heterologous virus vector in Nicotiana benthamiana plants, and purified under non-denaturing conditions. We found that RNAs in purified preparations were differentially enriched in sRNAs of 21 and to a much lesser extent of 22 nucleotides (nt) in length and of viral sequence (vsRNAs) when compared to those found in a control plant protein background bound to the nickel resin in the absence of HCPro, or in a purified alanine substitution HCPro mutant (HCPro mutB) control that lacked suppressor of silencing activity. In both controls, sRNAs were composed almost entirely by molecules of plant sequence, indicating that the resin-bound protein background had no affinity for vsRNAs, and also that HCPro mutB failed to bind to vsRNAs. Therefore, PVY HCPro suppressor activity correlated with its ability to bind to 21 and 22 nt vsRNAs. HCPro constituted at least 54 % of the total protein content in purified preparations and we were able to calculate its contribution to the 21 and the 22 nt pool of sRNAs present in the purified samples and its binding strength relative to the background. We also found that in the 21 nt vsRNAs of the HCPro preparation 5’ terminal adenines were overrepresented relative to the controls, but this was not observed in vsRNAs of other sizes, or of plant sequence.
Description34 p.-7 fig.-2 tab.-2 fig.supl.
Publisher version (URL)http://dx.doi.org/10.1128/JVI.00367-17
URIhttp://hdl.handle.net/10261/148269
DOI10.1128/JVI.00367-17
ISSN0022-538X
E-ISSN1098-5514
Appears in Collections:(CIB) Artículos
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